Development and characterization of a rat model for locally recurring mammary tumors: sensitivities to 5-fluoro-2'-deoxyuridine, adriamycin, and X-irradiation

Journal Article · Mon Aug 15 04:00:00 EDT 1988 · Cancer Res.; (United States)

OSTI ID:7071984

Welch, D R; Aeed, P A; Estrada, J

Local recurrence occurs in 4-47% of breast cancer patients and is often associated with development of metastatic foci and resistant cell populations. Thus, recurrent breast cancer indicates a poor prognosis for the patient. Local tumor-derived 13762NF rat mammary adenocarcinoma cell clone MTF7(T20) was injected into the inguinal mammary fat pad and allowed to grow before surgical excision. Individual locally growing (primary) tumors were removed and established in short-term tissue culture. Corresponding local recurrences were excised after regrowth and established in short-term tissue culture. All sublines were tested for in vitro sensitivities to 5-fluoro-2'-deoxyuridine, Adriamycin, and ionizing X-irradiation. Using a clonogenic colony formation assay, responses of individual sublines ranged from 85 to 1500 ng/ml for Adriamycin and 65 to 10,000 nM for FdUrd. Some recurrences were significantly more resistant while others were more sensitive than the corresponding primary tumor lines. All recurrences had smaller 90% lethal dose values than the corresponding parent or primary tumor in response to Adriamycin; whereas, to 5-fluoro-2'-deoxyuridine, 90% lethal dose values revealed that most lines were quite resistant. Statistically significant differences in radiation survival were observed only for lines LR1a and LR5 (more sensitive). There was no apparent correlation between sensitivities to chemotherapy agents or X-irradiation and experimental metastatic potential in LR sublines. These dose-response data indicate that locally recurrent tumors are frequently, but not always, different from the original primary tumor in response to chemotherapy agents and ionizing X-irradiation.

• Research Organization: Upjohn Company, Kalamazoo, MI (USA)
• OSTI ID: 7071984
• Journal Information: Cancer Res.; (United States), Journal Name: Cancer Res.; (United States) Vol. 48:16; ISSN CNREA
• Country of Publication: United States
• Language: English

Related Subjects

550600 -- Medicine
550603 -- Medicine-- External Radiation in Therapy-- (1980-)
560120* -- Radiation Effects on Biochemicals
Cells
& Tissue Culture
62 RADIOLOGY AND NUCLEAR MEDICINE
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
ANTI-INFECTIVE AGENTS
ANTIMETABOLITES
ANTIMICROBIAL AGENTS
ANTINEOPLASTIC DRUGS
AZINES
BIOLOGICAL MODELS
BODY
CARCINOMAS
COLONY FORMATION
COMBINED THERAPY
DISEASES
DOSE-RESPONSE RELATIONSHIPS
DRUGS
ELECTROMAGNETIC RADIATION
EXPERIMENTAL NEOPLASMS
FLUOROURACILS
FUDR
GLANDS
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
IONIZING RADIATIONS
MAMMALS
MAMMARY GLANDS
METASTASES
NEOPLASMS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC FLUORINE COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PYRIMIDINES
RADIATIONS
RADIOSENSITIVITY
RADIOSENSITIZERS
RATS
RIBOSIDES
RODENTS
SURVIVAL TIME
THERAPY
TUMOR CELLS
URACILS
VERTEBRATES
X RADIATION