Tissue-specific activity of the pro-opiomelanocortin gene promoter
Journal Article
·
· Mol. Cell. Biol.; (United States)
The pro-opiomelanocortin (POMC) gene is specifically expressed in corticotroph cells of the anterior pituitary. To define the POMC promoter sequences responsible for tissue-specific expression, we assessed POMC promoter activity by gene transfer into POMC-expressing pituitary tumor cells (AtT-20) and fibroblast L cells. The rat POMC promoter was only efficiently utilized and correctly transcribed in AtT-20 cells. 5'-End deletion analysis revealed two promoter regions for activity in AtT-20 cells. When tested by fusion to a heterologuous promoter, DNA fragments corresponding to both regions exhibited tissue-specific activity, suggesting the presence of at least two tissue-specific DNA sequence elements within the promoter. In summary, POMC promoter sequences from -480 to -34 base pairs appear sufficient to mimic the specificity of anterior pituitary expression.
- Research Organization:
- Laboratoire de Biologie Moleculaire des Eucaryotes, Institut de Recherches Cliniques de Montreal, Montreal, Quebec H2W 1R7 (CA)
- OSTI ID:
- 7070705
- Journal Information:
- Mol. Cell. Biol.; (United States), Journal Name: Mol. Cell. Biol.; (United States) Vol. 7:11; ISSN MCEBD
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550200* -- Biochemistry
550800 -- Morphology
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANIMALS
BODY
CELL DIFFERENTIATION
CLONING
DATA
DNA-CLONING
ENDOCRINE GLANDS
EXPERIMENTAL DATA
GENE OPERONS
GENE REGULATION
GENE REPRESSORS
GENES
GLANDS
HORMONES
HYBRIDIZATION
INFORMATION
L CELLS
MAMMALS
MOLECULAR BIOLOGY
NUCLEOPROTEINS
NUMERICAL DATA
ORGANIC COMPOUNDS
ORGANS
PEPTIDE HORMONES
PHENOTYPE
PITUITARY GLAND
PROTEINS
RATS
RODENTS
SPECIFICITY
TISSUES
TUMOR CELLS
VERTEBRATES
550800 -- Morphology
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANIMALS
BODY
CELL DIFFERENTIATION
CLONING
DATA
DNA-CLONING
ENDOCRINE GLANDS
EXPERIMENTAL DATA
GENE OPERONS
GENE REGULATION
GENE REPRESSORS
GENES
GLANDS
HORMONES
HYBRIDIZATION
INFORMATION
L CELLS
MAMMALS
MOLECULAR BIOLOGY
NUCLEOPROTEINS
NUMERICAL DATA
ORGANIC COMPOUNDS
ORGANS
PEPTIDE HORMONES
PHENOTYPE
PITUITARY GLAND
PROTEINS
RATS
RODENTS
SPECIFICITY
TISSUES
TUMOR CELLS
VERTEBRATES