Surface-enhanced resonance Raman scattering spectroscopy of photosystem II pigment-protein complexes

Journal Article · Thu Jun 09 04:00:00 EDT 1994 · Journal of Physical Chemistry; (United States)

DOI:https://doi.org/10.1021/j100074a031· OSTI ID:7069507

Picorel, R ^[1]; Chumanov, G; Cotton, T M ^[2]; Montoya, G ^[3]; Toon, S; Seibert, M ^[4]

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1. National Renewable Energy Lab., Golden, CO (United States) Estacion Experimental Aula Dei, Zaragoza (Spain)
2. Iowa State Univ., Ames, IA (United States)
3. Estacion Experimental Aula Dei, Zaragoza (Spain)
4. National Renewable Energy Lab., Golden, CO (United States)

Three different photosystem II (PSII) pigment-protein complexes (D1-D2-Cyt b[sub 559]-CP47, D1-D2-Cyt b[sub 559], and CP47) isolated from spinach were studied by surface-enhanced resonance Raman scattering (SERRS) spectroscopy. Surface-enhanced Raman scattering (SERS) is a distance sensitive (on a 5-10-[angstrom] scale) spectroscopic tool that can be used to examine structural properties of large biological molecules. It is demonstrated here that SERS can also be used to determine organizational relationships between different pigment-protein complexes. Strong SERRS spectra from the above PSII complexes before and after treatment with sodium dithionite were obtained on roughened Ag electrodes and in citrate-reduced Ag colloids. The D1-D2-Cyt b[sub 559] complex adsorbs with the Cyt b[sub 559] heme close to the surface in the colloid, whereas the complex adsorbs differently on the Ag electrode due to the differing surface properties of the two types of substrates. An analysis of the SERRS spectra led to the following conclusions: CP47 binds next to Cyt b[sub 559] in the D1-D2-Cyt b[sub 559]-CP47 complex and covers the heme, the Cyt b[sub 559] heme is located closer to one side of the complex (the stromal side in the intact thylakoid membrane), and both Chl and [beta]-carotene molecules are located closer to the opposite side of the complex. 37 refs., 7 figs.

• OSTI ID: 7069507
• Journal Information: Journal of Physical Chemistry; (United States), Journal Name: Journal of Physical Chemistry; (United States) Vol. 98:23; ISSN JPCHAX; ISSN 0022-3654
• Country of Publication: United States
• Language: English

Related Subjects

14 SOLAR ENERGY
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37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
400102 -- Chemical & Spectral Procedures
400201* -- Chemical & Physicochemical Properties
550200 -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
ALKALI METAL COMPOUNDS
CARBOXYLIC ACIDS
CHEMICAL REACTIONS
COLLOIDS
COMPLEXES
DISPERSIONS
ELECTRODES
ELEMENTS
FOOD
HEME
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
LASER SPECTROSCOPY
MAGNOLIOPHYTA
MAGNOLIOPSIDA
METALS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PHOTOCHEMICAL REACTIONS
PHOTOSYNTHESIS
PIGMENTS
PLANTS
PORPHYRINS
PROTEINS
RAMAN SPECTROSCOPY
SILVER
SODIUM COMPOUNDS
SPECTROSCOPY
SPINACH
SYNTHESIS
TRANSITION ELEMENTS
VEGETABLES