Liver cytochrome P-450E levels associated with chlorobiphenyl residues in starry flounder from San Francisco Bay
- Woods Hole Oceanographic Institution, MA (USA)
- Lawrence Livermore National Lab., CA (USA)
The objective of this study was to validate use of substrate and immunochemical probes to P-450E, to indicate effects of organic pollutants in fish from San Francisco Bay. Starry flounder (Platichthys stellatus) were collected during November 1986, and pieces of liver frozen on dry ice. In addition, starry flounder were held 2-3 months in recirculating water systems, then treated with {beta}-naphthoflavone (BNF) at 20 mg/ig i.p., and killed 5 days after treatment. The levels of microsomal P-450/mg protein in BNF-treated fish increased between two- and three-fold over those in control animals. The levels of ethoxyresorufin o-deethylase (EROD) activity per mg protein or per nmol total P-450 were also strongly induced. Immunoblot analysis of microsomes from control and treated fish with MAb 1-12-3 to P-450E from cup showed a single cross-reacting protein in the starry flounder, with an electrophoretic migration equal to a M{sub r} very close to that of P-450E, about 54,000. This counterpart of scup P-450E was very strongly increased in the treated animals. In contrast to the results with EROD activity, the levels of estradiol 2-hydroxylase activity/mg protein were unchanged by BNF treatment, consistent with other information indicating that estradiol 2-hydroxylase is not catalyzed by MC- or BNF-inducible P-450 forms. Average values for total P-450, EROD activity per mg protein and per nmole P-450, and P-450E content detected with MAb 1-12-3 of field-sampled fish are given. The levels of total P-450 and of EROD activity showed large individual variation within each group, yet indicated that a P-450E counterpart was present in most individuals from the field.
- OSTI ID:
- 7069029
- Report Number(s):
- CONF-8909236--
- Journal Information:
- Preprints of Papers Presented at National Meeting, Division of Water, Air and Waste Chemistry, American Chemical Society; (USA), Journal Name: Preprints of Papers Presented at National Meeting, Division of Water, Air and Waste Chemistry, American Chemical Society; (USA) Vol. 28:2; ISSN 0099-7293; ISSN ACWCA
- Country of Publication:
- United States
- Language:
- English
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540320* -- Environment
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560300 -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AROMATICS
BIOLOGICAL INDICATORS
BODY
CELL CONSTITUENTS
CHLORINATED AROMATIC HYDROCARBONS
CYTOCHROMES
DIGESTIVE SYSTEM
ELECTROPHORESIS
ENZYME ACTIVITY
ENZYMES
GLANDS
HALOGENATED AROMATIC HYDROCARBONS
LIVER
MICROSOMES
ORGANIC CHLORINE COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
ORGANOIDS
ORGANS
OXIDOREDUCTASES
PACIFIC OCEAN
PIGMENTS
POLLUTION
PROTEINS
RIBOSOMES
SAN FRANCISCO BAY
SEAS
SURFACE WATERS
TOXICITY
WATER POLLUTION