Enzymatic synthesis of a novel acetylated neutral lipid (related to platelet activating factor, PAF) by acyl-CoA: 1-alkyl-2-acetyl-sn-glycerol acyltransferase

Conference · Fri May 01 04:00:00 EDT 1987 · FASEB (Federation of American Societies for Experimental Biology) Journal; (USA)

OSTI ID:7068618

Kawasaki, Tomio; Synder, F ^[1]

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1. Oak Ridge Associated Univ., TN (USA)

Recent data from our laboratory have described the complete enzymatic steps for the de novo synthesis of alkylacetylglycerols and their subsequent conversion to PAF. Experiments are reported here to show that the alkylacetylglycerols also are a substrate for an acyltransferase. Homogenates of HL-60 cells produced labeled 1-alkyl-2-acetyl-3-acyl-sn-glycerols from 1-({sup 3}H)hexadecyl-2-acetyl-sn-glycerol, 1-alkyl-2-({sup 3}H)acetyl-sn-glycerol, or (1-{sup 14}C)linoleic acid when incubated with CoA (0.1 mM), ATP (10 mM), and Mg{sup 2+} (95 mM). Formation of labeled alkylacetylacyl-glycerols by the acyltransferase required CoA, ATP, and Mg{sup 2+}. The labeled alkylacetylacylglycerol produced had an identical R{sub f} to that of an authenic standard; after treatment with pancreatic lipase, the sn-3 acyl moiety was hydrolyzed to regenerate the original 1-alkyl-2-acetyl-sn-glycerol precursor. (1-{sup 14}C)Linoleic acid was an excellent substrate for the acylation step, whereas (1-{sup 14}C)oleic acid was barely utilized. Kinetic properties and related characteristics of this enzyme that forms the acetyl analog of a triglyceride will be presented. Although the function of this new type of acetylated neutral lipid class is presently unknown, it could serve as a potential precursor reservoir in the de novo route of PAF synthesis.

• OSTI ID: 7068618
• Report Number(s): CONF-870644--
• Conference Information: Journal Name: FASEB (Federation of American Societies for Experimental Biology) Journal; (USA) Journal Volume: 46:6
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ACETYLATION
ACYLATION
ALCOHOLS
ALKALINE EARTH METAL COMPOUNDS
ANIMAL CELLS
ATP
BIOCHEMICAL REACTION KINETICS
BIOSYNTHESIS
CARBON 14 COMPOUNDS
CARBOXYLESTERASES
CARBOXYLIC ACIDS
CATIONS
CHARGED PARTICLES
CHEMICAL REACTIONS
COENZYMES
DECOMPOSITION
ENZYMES
ESTERASES
GLYCEROL
HYDROGEN COMPOUNDS
HYDROLASES
HYDROLYSIS
HYDROXY COMPOUNDS
IONS
ISOTOPE APPLICATIONS
KINETICS
LABELLED COMPOUNDS
LINOLEIC ACID
LIPASES
LIPIDS
LYSIS
MAGNESIUM COMPOUNDS
MONOCARBOXYLIC ACIDS
NUCLEOTIDES
OLEIC ACID
ORGANIC ACIDS
ORGANIC COMPOUNDS
PRECURSOR
REACTION KINETICS
SOLVOLYSIS
SYNTHESIS
TRACER TECHNIQUES
TRANSFERASES
TRITIUM COMPOUNDS