Radiation inactivation analysis of fusion and hemolysis by vesicular stomatitis virus

Journal Article · Fri Apr 01 04:00:00 EST 1988 · Virology; (United States)

DOI:https://doi.org/10.1016/0042-6822(88)90304-2· OSTI ID:7067573

Bundo-Morita, K; Gibson, S; Lenard, J

Radiation inactivation analysis was used to determine the size of the functional unit responsible for fusion of vesicular stomatitis virus (VSV) with cardiolipin or phosphatidylcholine-phosphatidylethanolamine (1:1) liposomes, and for VSV-induced hemolysis. When radiation-insensitive background values were subtracted, the calculated functional units for all three activities were similar, ranging from 866 to 957 kDa, equivalent to about 15 G protein molecules. This is in striking contrast to results of similar studies with influenza and Sendai viruses, in which the functional unit corresponded in size to a single fusion protein monomer, and suggests that VSV fusion may occur by a different mechanism.

• Research Organization: Univ. of Medicine and Dentistry of New Jersey, Piscataway (USA)
• OSTI ID: 7067573
• Journal Information: Virology; (United States), Journal Name: Virology; (United States) Vol. 163:2; ISSN VIRLA
• Country of Publication: United States
• Language: English

Related Subjects

560130* -- Radiation Effects on Microorganisms
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
CELL CONSTITUENTS
HEMOLYSIS
KINETICS
LIPOSOMES
LYSIS
MEMBRANE PROTEINS
MICROORGANISMS
MOLECULAR WEIGHT
ORGANIC COMPOUNDS
ORGANOIDS
PARASITES
PATHOLOGICAL CHANGES
PROTEINS
RADIATION EFFECTS
REACTION KINETICS
VIRUSES