Role of the Bradyrhizobium japonicum ntrC gene product in differential regulation of the glutamine synthetase II gene (glnII)
Journal Article
·
· Journal of Bacteriology; (USA)
OSTI ID:7067562
- Michigan State Univ., East Lansing (USA)
We isolated the ntrC gene from Bradyrhizobium japonicum, the endosymbiont of soybean (Glycine max), and examined its role in regulating nitrogen assimilation. Two independent ntrC mutants were constructed by gene replacement techniques. One mutant was unable to produce NtrC protein, while the other constitutively produced a stable, truncated NtrC protein. Both ntrC mutants were unable to utilize potassium nitrate as a sole nitrogen source. In contrast to wild-type B. japonicum, the NtrC null mutant lacked glnII transcripts in aerobic, nitrogen-starved cultures. However, the truncated-NtrC mutant expressed glnII in both nitrogen-starved and nitrogen-excess cultures. Both mutants expressed glnII under oxygen-limited culture conditions and in symbiotic cells. These results suggest that nitrogen assimilation in B. japonicum is regulated in response to both nitrogen limitation and oxygen limitation and that separate regulatory networks exist in free-living and symbiotic cells.
- OSTI ID:
- 7067562
- Journal Information:
- Journal of Bacteriology; (USA), Journal Name: Journal of Bacteriology; (USA) Vol. 170:12; ISSN JOBAA; ISSN 0021-9193
- Country of Publication:
- United States
- Language:
- English
Similar Records
Differential transcription of the two glutamine synthetase gene of Bradyrhizobium japonicum
Enhanced nitrogen fixation in a Rhizobium etli ntrC mutant that overproduces the Bradyrhizobium japonicum symbiotic terminal oxidase cbb{sub 3}
(Iron regulation of gene expression in the Bradyrhizobium japonicum/soybean symbiosis)
Journal Article
·
Mon Nov 30 23:00:00 EST 1987
· J. Bacteriol.; (United States)
·
OSTI ID:5381993
Enhanced nitrogen fixation in a Rhizobium etli ntrC mutant that overproduces the Bradyrhizobium japonicum symbiotic terminal oxidase cbb{sub 3}
Journal Article
·
Sat May 01 00:00:00 EDT 1999
· Applied and Environmental Microbiology
·
OSTI ID:346830
(Iron regulation of gene expression in the Bradyrhizobium japonicum/soybean symbiosis)
Technical Report
·
Tue Dec 31 23:00:00 EST 1991
·
OSTI ID:5312051