In vitro and in vivo properties of human/mouse chimeric monoclonal antibody specific for common acute lymphocytic leukemia antigen

Journal Article · Fri Jun 01 04:00:00 EDT 1990 · Journal of Nuclear Medicine; (USA)

OSTI ID:7063271

Saga, T; Endo, K; Koizumi, M; Kawamura, Y; Watanabe, Y; Konishi, J; Ueda, R; Nishimura, Y; Yokoyama, M; Watanabe, T ^[1]

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1. Kyoto Univ. School of Medicine (Japan)

A human/mouse chimeric monoclonal antibody specific for a common acute lymphocytic leukemia antigen was efficiently obtained by ligating human heavy-chain enhancer element to the chimeric heavy- and light-chain genes. Cell binding and competitive inhibition assays of both radioiodine and indium-111- (111In) labeled chimeric antibodies demonstrated in vitro immunoreactivity identical with that of the parental murine monoclonal antibodies. The biodistribution of the radiolabeled chimeric antibody in tumor-bearing nude mice was similar to that of the parental murine antibody. Tumor accumulation of radioiodinated parental and chimeric antibodies was lower than that of {sup 111}In-labeled antibodies, probably because of dehalogenation of the radioiodinated antibodies. Indium-111-labeled chimeric antibody clearly visualized xenografted tumor. These results suggest that a human/mouse chimeric antibody can be labeled with {sup 111}In and radioiodine without the loss of its immunoreactivity, and that chimeric antibody localizes in vivo in the same way as the parental murine antibody.

• OSTI ID: 7063271
• Journal Information: Journal of Nuclear Medicine; (USA), Journal Name: Journal of Nuclear Medicine; (USA) Vol. 31:6; ISSN 0161-5505; ISSN JNMEA
• Country of Publication: United States
• Language: English

Related Subjects

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62 RADIOLOGY AND NUCLEAR MEDICINE
ANIMALS
ANTIBODIES
ANTIGENS
BETA DECAY RADIOISOTOPES
BIOLOGICAL LOCALIZATION
CHIMERAS
DAYS LIVING RADIOISOTOPES
DISEASES
DISTRIBUTION
ELECTRON CAPTURE RADIOISOTOPES
EXPERIMENTAL NEOPLASMS
GENES
HEMIC DISEASES
IMMUNE REACTIONS
IMMUNE SYSTEM DISEASES
IN VITRO
IN VIVO
INDIUM 111
INDIUM ISOTOPES
INTERMEDIATE MASS NUCLEI
IODINE ISOTOPES
ISOMERIC TRANSITION ISOTOPES
ISOTOPES
LABELLING
LEUKEMIA
MAMMALS
MAN
MICE
MINUTES LIVING RADIOISOTOPES
MONOCLONAL ANTIBODIES
MOSAICISM
NEOPLASMS
NUCLEI
ODD-EVEN NUCLEI
PRIMATES
RADIOISOTOPES
RODENTS
SPECIFICITY
TISSUE DISTRIBUTION
VERTEBRATES