The complete sequence and promoter activity of the human A-raf-1 gene (ARAF1)

Journal Article · Tue Mar 01 00:00:00 EST 1994 · Genomics; (United States)

DOI:https://doi.org/10.1006/geno.1994.1125· OSTI ID:7062376

Lee, J E; Beck, T W; Brennscheidt, U; Rapp, U R ^[1]; DeGennaro, L J ^[2]

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1. National Cancer Institute, Frederick, MD (United States)
2. Univ. of Massachusetts Medical School, Worcester, MA (United States)

The raf proto-oncogenes encode cytoplasmic protein serine/threonine kinases, which play a critical role in cell growth and development. One of these, A-raf-1 (human gene symbol, ARAF1), which is predominantly expressed in mouse urogenital tissues, has been mapped to an evolutionarily conserved linkage group composed of ARAF1, SYN1, TIMP, and properdin located at human chromosome Xp11.2. The authors have isolated human genomic DNA clones containing the expressed gene (ARAF1) on the X chromosome and a pseudogene (ARAF2) on chromosome 7p12-q11.21. Analysis of the nucleotide sequence from the ARAF1 genomic clones demonstrated that it consists of 16 exons encoded by minimally 10,776 nucleotides. The major transcriptional start site (+1) was determined by RNase protection and primer extension assays. Promoter activity was confirmed by functional assays using DNA fragments fused to a CAT reporter gene. The ARAF1 minimal promoter, located between nucleotides -59 and +93, has a low G + C content and lacks consensus TATA and Inr sequences but shows sequence similarity at position -1 to the E box that is known to interact with USF and TFII-I transcription factors. 65 refs., 7 figs., 1 tab.

• OSTI ID: 7062376
• Journal Information: Genomics; (United States), Vol. 20:1; ISSN 0888-7543
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
HUMAN CHROMOSOME 7
GENETIC MAPPING
HUMAN X CHROMOSOME
ONCOGENES
DNA SEQUENCING
PHOSPHOTRANSFERASES
CHROMOSOMES
ENZYMES
GENES
HETEROCHROMOSOMES
HUMAN CHROMOSOMES
MAPPING
ORGANIC COMPOUNDS
PHOSPHORUS-GROUP TRANSFERASES
PROTEINS
STRUCTURAL CHEMICAL ANALYSIS
TRANSFERASES
X CHROMOSOME
550400* - Genetics