A nude rat model for neutron capture therapy of human intracerebral melanoma

Journal Article · Wed Mar 30 00:00:00 EST 1994 · International Journal of Radiation Oncology, Biology and Physics; (United States)

DOI:https://doi.org/10.1016/0360-3016(94)90481-2· OSTI ID:7061563

Barth, R F; Matalka, K Z; Bailey, M Q; Staubus, A E; Soloway, A H; Moeschberger, M L ^[1]; Coderre, J A ^[2]; Rofstad, E K ^[3]

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1. Ohio State Univ., Columbus, OH (United States)
2. Brookhaven National Lab., Upton, NY (United States)
3. Norwegian Radium Hospital, Oslo (Norway)

The present study was carried out to determine the efficacy of Boron Neutron Capture Therapy (BNCT) for intracerebral melanoma using nude rats, the human melanoma cell line MRA 27, and boronophenylalanine as the capture agent. MRA 27 cells (2 [times] 10[sup 5]) were implanted intracerebrally, and 30 days later, 120 mg of [sup 10]B-L-BPA were injected intraperitoneally into nude rats. Thirty days following implantation, tumor bearing rats were irradiated at the Brookhaven Medical Research Reactor. Six hours following administration of BPA, tumor, blood, and normal brain boron-10 levels were 23.7, 9.4, and 8.4 [mu]g/g respectively. Median survival time of untreated rats was 44 days compared to 76 days and 93 days for those receiving physical doses of 2.73 Gy and 3.64 Gy, respectively. Rats that have received both [sup 10]B-BPA and physical doses of 1.82, 2.73, or 3.64 Gy had median survival times of 170, 182, and 262 days, respectively. Forty percent of rats that had received the highest tumor dose (10.1 Gy) survived for > 300 days and in a replicate experiment 21% of the rats were longterm survivors (>220 days). Animals that received 12 Gy in a single dose or 18 Gy fractionated (2 Gy [times] 9) of gamma photons from a [sup 137]Cs source had median survival times of 86 and 79 days, respectively, compared to 47 days for untreated animals. Histopathologic examination of the brains of longterm surviving rats, euthanized at 8 or 16 months following BNCT, showed no residual tumor, but dense accumulations of melanin laden macrophages and minimal gliosis were observed. Significant prolongations in median survival time were noted in nude rats with intracerebral human melanoma that had received BNCT, thereby suggesting therapeutic efficacy. Large animal studies should be carried out to further assess BNCT of intracerebral melanoma before any human trials are contemplated. 49 refs., 7 figs., 2 tabs.

• OSTI ID: 7061563
• Journal Information: International Journal of Radiation Oncology, Biology and Physics; (United States), Vol. 28:5; ISSN 0360-3016
• Country of Publication: United States
• Language: English

Related Subjects

62 RADIOLOGY AND NUCLEAR MEDICINE
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BRAIN
NEUTRON CAPTURE THERAPY
MELANOMAS
BIOLOGICAL MODELS
MICE
ANIMALS
BODY
CARCINOMAS
CENTRAL NERVOUS SYSTEM
DISEASES
EPITHELIOMAS
MAMMALS
MEDICINE
NEOPLASMS
NERVOUS SYSTEM
NEUTRON THERAPY
NUCLEAR MEDICINE
ORGANS
RADIOLOGY
RADIOTHERAPY
RODENTS
THERAPY
VERTEBRATES
550603* - Medicine- External Radiation in Therapy- (1980-)
560152 - Radiation Effects on Animals- Animals
560300 - Chemicals Metabolism & Toxicology