Differential carcinogenic effects of intraperitoneal initiation with 7,12-dimethylbenz(a)anthracene or urethane and topical promotion with 12-O-tetradecanoylphorbol-13-acetate in skin and internal tissues of female SENCAR and BALB/c mice

Journal Article · Mon Sep 01 04:00:00 EDT 1986 · Environ. Health Perspect.; (United States)

DOI:https://doi.org/10.1289/ehp.866861· OSTI ID:7057853

Ward, J M; Rehm, S; Devor, D; Hennings, H; Wenk, M L

Groups of female SENCAR or BALB/c mice were initiated once intraperitoneally with 300 ..mu..g/mouse of 7,12-dimethylbenz(a) anthracene (DMBA) or 20 mg/mouse of urethane at 7 weeks of age. Beginning one week later, mice received topically applied acetone or 12-O-tetradecanoylphorbol-13-acetate (TPA), once weekly, at 2.5 ..mu..g/mouse for weeks 1 through 6 and 1.25 ..mu..g/mouse for weeks 7 through 52. The skin lesions were evaluated clinically. A complete necropsy was performed on all mice at week 52. SENCAR mice exposed to DMBA/TPA and urethane/TPA had more skin tumors than SENCAR mice exposed to DMBA or urethane alone and more than BALB/c mice in any treatment group. Of all skin carcinomas diagnosed histologically in DMBA/TPA-exposed mice, less than one-third had been identified clinically while the mice were alive. Most of the carcinomas arose within papillomas. BALB/c mice developed more vascular and uterine tumors than did SENCAR mice injected with DMBA and more lung and vascular tumors than did SENCAR mice injected with urethane. TPA exposure after treatment with either initiator had no significant effect on internal tumor development in either SENCAR or BALB/c mice.

• Research Organization: NIH, Frederick, MD
• OSTI ID: 7057853
• Journal Information: Environ. Health Perspect.; (United States), Journal Name: Environ. Health Perspect.; (United States) Vol. 68; ISSN EVHPA
• Country of Publication: United States
• Language: English

Related Subjects

560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
AROMATICS
BIOLOGICAL EFFECTS
BODY
CARBAMATES
CARBONIC ACID DERIVATIVES
CARBOXYLIC ACID SALTS
CARCINOGENESIS
CARCINOGENS
CARCINOMAS
CONDENSED AROMATICS
DIMETHYLBENZANTHRACENE
DISEASE INCIDENCE
DISEASES
ESTERS
FEMALE GENITALS
FEMALES
GLANDS
LUNGS
MAMMALS
MAMMARY GLANDS
MICE
NEOPLASMS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PATHOGENESIS
PHORBOL ESTERS
PROMOTERS
RESPIRATORY SYSTEM
RODENTS
SKIN
TUMOR PROMOTERS
URETHANE
UTERUS
VASCULAR DISEASES
VERTEBRATES