Hepatic cadmium, metal-binding proteins and bioaccumulation in bluegills exposed to aqueous cadmium

Journal Article · Fri Apr 01 04:00:00 EST 1994 · Environmental Toxicology and Chemistry; (United States)

DOI:https://doi.org/10.1002/etc.5620130403· OSTI ID:7051088

Cope, W G; Atchison, G J ^[1]; Wiener, J G ^[2]

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1. Iowa State Univ., Ames, IA (United States). Dept. of Animal Ecology
2. National Fisheries Contaminant Research Center, La Crosse, WI (United States). National Biological Survey

The authors examined sublethal response of juvenile bluegills Lepomis macrochirus to aqueous cadmium in two 28-d tests in an intermittent-flow diluter. The experimental design was completely randomized, with two replicates in each of eight treatments. Cadmium did not affect the growth of test fish. the mean whole-body concentrations of Cd in exposed fish were 1.8- to 44-fold those in controls in the two tests. Mean concentrations of hepatic nonthionein cytosolic Cd (not bound by metal-binding proteins, MBP) in all Cd treatment greatly exceeded those in controls, and mean concentrations of hepatic MBP in all treatments except one exceeded those in controls. Nonthionein cytosolic cd, hepatic MBP, and whole-body Cd in bluegills were linearly related to exposure concentrations within the range 0 to 20 [mu]g Cd per liter. Much of the total Cd- binding capacity of hepatic MBP per fish was occupied by Cd after the 28-d exposure, although additional Cd-binding capacity remained unoccupied by Cd in fish in all treatments. The mean total Cd-binding capacity of hepatic MBP per fish, which ranged from 1.7 to 14 nmol Cd in test 1 and from 0.8 to 24 nmol Cd in test 2, increased in a concentration-response manner at exposure concentrations below 13 [mu]g/L. Nonthionein cytosolic Cd was the most sensitive indicator of Cd exposure, based on an LOEC of 0.8 [mu]g Cd per liter.

• OSTI ID: 7051088
• Journal Information: Environmental Toxicology and Chemistry; (United States), Journal Name: Environmental Toxicology and Chemistry; (United States) Vol. 13:4; ISSN 0730-7268; ISSN ETOCDK
• Country of Publication: United States
• Language: English

Related Subjects

560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
AQUATIC ORGANISMS
BIOLOGICAL ACCUMULATION
BIOSYNTHESIS
CADMIUM
ELEMENTS
FISHES
METABOLISM
METALLOPROTEINS
METALS
ORGANIC COMPOUNDS
PROTEINS
SENSITIVITY
SYNTHESIS
TOXICITY
VERTEBRATES