In vivo somatostatin, vasopressin, and oxytocin synthesis in diabetic rat hypothalamus
- Univ. of Pittsburgh School of Medicine, PA (USA)
The in vivo labeling of somatostatin-14, somatostatin-28, arginine vasopressin, and oxytocin was studied in rat hypothalamus after third ventricular administration of (35S)cysteine to streptozotocin-diabetic and normal rats. Immunoreactive somatostatin levels in hypothalamus were unaffected by diabetes, as was the incorporation of (35S)cysteine into hypothalamic somatostatin-14 and somatostatin-28. In contrast, immunoreactive vasopressin levels in hypothalamus and posterior pituitary (and oxytocin levels in posterior pituitary) were below normal in diabetic rats. Moreover, (35S)cysteine incorporation into hypothalamic vasopressin and oxytocin (probably mainly in the paraventricular nucleus because of its proximity to the third ventricular site of label injection) was significantly above normal. The increments in vasopressin and oxytocin labeling were reversed by insulin administration. In vivo cysteine specific activity and the labeling of acid-precipitable protein did not differ between normal and diabetic animals; effects of diabetes on vasopressin and oxytocin labeling were therefore not caused by simple differences in cysteine specific activity. These results suggest that diabetes (1) does not influence the production of somatostatin peptides in hypothalamus but (2) stimulates the synthesis of vasopressin and oxytocin. For vasopressin at least, the increase in synthesis may be a compensatory response to the known increase in its secretion that occurs in uncontrolled diabetes.
- OSTI ID:
- 7043944
- Journal Information:
- American Journal of Physiology; (USA), Vol. 258; ISSN 0002-9513
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
DIABETES MELLITUS
PATHOGENESIS
OXYTOCIN
BIOSYNTHESIS
SOMATOSTATIN
VASOPRESSIN
CYSTEINE
GLUCOSE
HYPOTHALAMUS
IN VIVO
LABELLING
PITUITARY GLAND
PROTEINS
RATS
SULFUR 35
TRACER TECHNIQUES
ALDEHYDES
AMINO ACIDS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BODY
BRAIN
CARBOHYDRATES
CARBOXYLIC ACIDS
CENTRAL NERVOUS SYSTEM
DAYS LIVING RADIOISOTOPES
DISEASES
ENDOCRINE DISEASES
ENDOCRINE GLANDS
EVEN-ODD NUCLEI
GLANDS
HEXOSES
HORMONES
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
MAMMALS
METABOLIC DISEASES
MONOSACCHARIDES
NERVOUS SYSTEM
NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
ORGANS
PEPTIDE HORMONES
PITUITARY HORMONES
RADIOISOTOPES
RODENTS
SACCHARIDES
SULFUR ISOTOPES
SYNTHESIS
THIOLS
VERTEBRATES
550901* - Pathology- Tracer Techniques