Intrathymic radioresistant stem cells follow an IL-2/IL-2R pathway during thymic regeneration after sublethal irradiation
Journal Article
·
· Journal of Immunology; (USA)
OSTI ID:7043124
- National Institutes of Health, Bethesda, MD (USA)
Sublethally irradiated mice undergo thymic regeneration which follows a phenotypic pattern of events similar to that observed during normal fetal development. Thymic regeneration after irradiation is the product of a limited pool of intrathymic radioresistant stem cells undergoing simultaneous differentiation. We show that in this model of T cell development, thymic regeneration follows a pathway in which the IL-2R is transiently expressed on CD4-/CD8- cells. IL-2R expression occurred during the exponential growth period of thymic regeneration, and IL-2R blocking prevented this explosive growth. Flow cytometry analysis revealed that the IL-2R blockade affected primarily the development of the immature CD3-/CD4-/CD8- (triple negative) cells and their ability to generate CD3+/CD4+/CD8+ or CD3+/CD4+/CD8- and CD3+/CD4-/CD8+ thymocytes. Thus, our findings demonstrate that blocking of the IL-2R resulted in an arrest in proliferation and differentiation by intrathymic radioresistant stem cells, indicating that the IL-2/IL-2R pathway is necessary for the expansion of immature triple negative T cells.
- OSTI ID:
- 7043124
- Journal Information:
- Journal of Immunology; (USA), Journal Name: Journal of Immunology; (USA) Vol. 144:10; ISSN JOIMA; ISSN 0022-1767
- Country of Publication:
- United States
- Language:
- English
Similar Records
In vivo administration of IL-1 induces thymic hypoplasia and increased levels of serum corticosterone
Cell proliferation and thymocyte subset reconstitution in sublethally irradiated mice: Compared kinetics of endogenous and intrathymically transferred progenitors
Kinetics of mature T-cell development in the thymus
Journal Article
·
Thu Sep 01 00:00:00 EDT 1988
· J. Immunol.; (United States)
·
OSTI ID:7091540
Cell proliferation and thymocyte subset reconstitution in sublethally irradiated mice: Compared kinetics of endogenous and intrathymically transferred progenitors
Journal Article
·
Sat Jul 01 00:00:00 EDT 1989
· Proc. Natl. Acad. Sci. USA; (United States)
·
OSTI ID:5696612
Kinetics of mature T-cell development in the thymus
Journal Article
·
Sat Mar 31 23:00:00 EST 1990
· Proceedings of the National Academy of Sciences of the United States of America; (USA)
·
OSTI ID:6896801
Related Subjects
560152* -- Radiation Effects on Animals-- Animals
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
ANTIGENS
BIOLOGICAL MATERIALS
BIOLOGICAL PATHWAYS
BIOLOGICAL RECOVERY
BIOLOGICAL REGENERATION
BLOOD
BLOOD CELLS
BODY
BODY FLUIDS
CELL DIFFERENTIATION
CELL DIVISION
CELL FLOW SYSTEMS
CONNECTIVE TISSUE CELLS
GROWTH FACTORS
LEUKOCYTES
LYMPHATIC SYSTEM
LYMPHOCYTES
LYMPHOKINES
MAMMALS
MATERIALS
MEMBRANE PROTEINS
MICE
MITOGENS
ORGANIC COMPOUNDS
ORGANS
PROTEINS
RADIOSENSITIVITY
RECEPTORS
RECOVERY
RODENTS
SOMATIC CELLS
STEM CELLS
THYMOCYTES
THYMUS
VERTEBRATES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
ANTIGENS
BIOLOGICAL MATERIALS
BIOLOGICAL PATHWAYS
BIOLOGICAL RECOVERY
BIOLOGICAL REGENERATION
BLOOD
BLOOD CELLS
BODY
BODY FLUIDS
CELL DIFFERENTIATION
CELL DIVISION
CELL FLOW SYSTEMS
CONNECTIVE TISSUE CELLS
GROWTH FACTORS
LEUKOCYTES
LYMPHATIC SYSTEM
LYMPHOCYTES
LYMPHOKINES
MAMMALS
MATERIALS
MEMBRANE PROTEINS
MICE
MITOGENS
ORGANIC COMPOUNDS
ORGANS
PROTEINS
RADIOSENSITIVITY
RECEPTORS
RECOVERY
RODENTS
SOMATIC CELLS
STEM CELLS
THYMOCYTES
THYMUS
VERTEBRATES