Peculiarities of RFLP of highly repetitive DNA in crow genomes
- Institute of Biology and Soil Sciences, Vladivostok (Russian Federation)
- Institute of Cytology and Genetics, Novosibirsk (Russian Federation)
We present a study of the structural organization of highly repetitive DNA in genomes of hooded crow Corvus cornix L., carrion crow C. corone L., and jungle crow C. macrorhynchos Wagl. RFLP and blot-hybridization with {sup 32}P-labeled Msp I fragment from hooded crow nDNA suggest the interspecific structural conservatism of the most repetitive DNA. The family of repeats we studied had tandem organization and the same (210 bp) period of reiteration for a set of restriction enzymes. However, in parallel to the general similarity of restriction patterns, there are species-specific peculiarities. The repetitive family revealed (Alu I, BsuR I, and Msp I fragments) has quantitative RFLP of nDNA and interspecific differences in the extent of the multimer {open_quotes}ladder{close_quotes} pattern of Msp I fragments. The latter is more pronounced in nDNA of carrion crow than in that of phylogenetically distant jungle crow and closely related hooded crow. This suggests a recent amplification event for highly organized homological repeats in crow genomes. 10 refs., 2 figs.
- OSTI ID:
- 70413
- Journal Information:
- Russian Journal of Genetics, Vol. 31, Issue 2; Other Information: PBD: Feb 1995; TN: Translated from Genetika; 31: No. 2, 174-179(1995)
- Country of Publication:
- United States
- Language:
- English
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