Novel tests for rapid detection of insecticide resistance in mosquito vectors. Final report, 15 June 1985-31 January 1989
This project is concerned with the formulation of novel diagnostic tests for the detection of organophosphate (OP) resistance in individual mosquitoes. Emphasis was placed on the mechanisms of detoxification by esterases and of insensitive acetycholinesterase. Seven diagnostic tests have resulted from this research to date. Five concern esterases, and two insensitive acetylcholinesterase. An eighth procedure concerns the qualification of proteins in single insects and is used in conjunction with other tests to account for variations in the size of the test insect. Three of the tests developed for esterases (FP/Est, NC/Est and MT/Est) utilize the ability of these enzymes to hydrolyze naphthyl acetate, and two (Dot-Blot/Est and ELISA/Est) involve immunological reactions. The tests for insensitive acetylcholinesterase (NC/AChE and MT/AChE) utilize the differences in inhibitory properties of insecticides on insensitive and normal enzymes.
- Research Organization:
- California Univ., Riverside, CA (United States). Dept. of Entomology
- OSTI ID:
- 7037013
- Report Number(s):
- AD-B-135625/2/XAB; CNN: DAMD17-85-C-5170
- Country of Publication:
- United States
- Language:
- English
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