Solid-state /sup 13/C nuclear magnetic resonance spectroscopy of simultaneously metabolized acetate and phenol in a soil Pseudomonas sp
An investigation was made of the concentration-dependent primary and secondary substrate relationships in the simultaneous metabolism of the ubiquitous pollutant phenol and the naturally occurring substrate acetate by a Pseudomonas sp. soil isolate capable of utilizing either substance as a sole source of carbon and energy. In addition to conventional analytical techniques, solid-state /sup 13/C nuclear magnetic resonance spectroscopy was used to follow the cellular distribution of (1-/sup 13/C)acetate in the presence of unlabeled phenol. These results suggest that, when phenol is present as the primary substrate, acetate is preferentially shuttled into fatty acyl chain synthesis, whereas phenol carbon is funnelled into the tricarboxylic acid cycle. Thus, simultaneous use of a xenobiotic compound and a natural substrate apparently does occur, and the relative concentrations of the two substrates do influence the rate and manner in which the compounds are utilized. These results also demonstrate the unique advantage of using solid-state nuclear magnetic resonance techniques combined with /sup 13/C labeling of specific sites in substrates when doing microbial degradation studies. In this work, the entire cellular biomass was examined directly without extensive extraction, fractionation, or isolation of subcellular units; thus, there is no uncertainty about chemical alteration of substrate metabolites as a result of these often harsh treatments.
- Research Organization:
- Florida State Univ., Tallahassee
- OSTI ID:
- 7034306
- Journal Information:
- Appl. Environ. Microbiol.; (United States), Vol. 53:1
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
ACETATES
BIODEGRADATION
PHENOL
PSEUDOMONAS
METABOLISM
CARBON 13
KREBS CYCLE
NMR SPECTRA
NUCLEAR MAGNETIC RESONANCE
SOILS
AROMATICS
BACTERIA
CARBON ISOTOPES
CARBOXYLIC ACID SALTS
CHEMICAL REACTIONS
DECOMPOSITION
EVEN-ODD NUCLEI
HYDROXY COMPOUNDS
ISOTOPES
LIGHT NUCLEI
MAGNETIC RESONANCE
MICROORGANISMS
NUCLEI
ORGANIC COMPOUNDS
PHENOLS
RESONANCE
SPECTRA
STABLE ISOTOPES
550501* - Metabolism- Tracer Techniques