DNA repair rates mapped along the human PGK1 gene at nucleotide resolution
Journal Article
·
· Science (Washington, D.C.); (United States)
- Institute of the City of Hope, Duarte, CA (United States)
The repair of cyclobutane pyrimidine dimers (CPDs), DNA lesions induced by ultraviolet light, was studied at nucleotide resolution. Human fibroblasts were irradiated with ultraviolet light and allowed to repair. The DNA was enzymatically cleaved at the CPDs, and the induced breaks along the promoter and exon 1 of the PGK1 gene were mapped by ligation-mediated polymerase chain reaction. Repair rates within the nontranscribed strand varied as much as 15-fold, depending on nucleotide position. Preferential repair of the transcribed strand began just downstream of the transcription start site but was most pronounced beginning at nucleotide +140 in exon 1. The promoter contained two slowly repaired regions that coincided with two transcription factor binding sites.
- OSTI ID:
- 7033882
- Journal Information:
- Science (Washington, D.C.); (United States), Journal Name: Science (Washington, D.C.); (United States) Vol. 263:5152; ISSN SCIEAS; ISSN 0036-8075
- Country of Publication:
- United States
- Language:
- English
Similar Records
Repair rate maps of cyclopyrimidine dimers along the human PFG-1 gene
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Sites of preferential induction of cyclobutane pyrimidine dimers in the nontranscribed strand of lacI correspond with sites of UV-induced mutation in Escherichia coli
Journal Article
·
Fri Dec 30 23:00:00 EST 1994
· Environmental and Molecular Mutagenesis
·
OSTI ID:88811
Strand specific DNA repair in different stages of the cell cycle
Conference
·
Thu Dec 31 23:00:00 EST 1992
· Cytometry (Baltimore); (United States)
·
OSTI ID:6827792
Sites of preferential induction of cyclobutane pyrimidine dimers in the nontranscribed strand of lacI correspond with sites of UV-induced mutation in Escherichia coli
Journal Article
·
Tue Jun 25 00:00:00 EDT 1991
· Journal of Biological Chemistry; (United States)
·
OSTI ID:5506415
Related Subjects
550400 -- Genetics
560120* -- Radiation Effects on Biochemicals
Cells
& Tissue Culture
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
CHEMICAL RADIATION EFFECTS
DNA
DNA REPAIR
ELECTROMAGNETIC RADIATION
GENETIC EFFECTS
GENETIC RADIATION EFFECTS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PYRIMIDINE DIMERS
RADIATION EFFECTS
RADIATIONS
REPAIR
ULTRAVIOLET RADIATION
560120* -- Radiation Effects on Biochemicals
Cells
& Tissue Culture
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
CHEMICAL RADIATION EFFECTS
DNA
DNA REPAIR
ELECTROMAGNETIC RADIATION
GENETIC EFFECTS
GENETIC RADIATION EFFECTS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PYRIMIDINE DIMERS
RADIATION EFFECTS
RADIATIONS
REPAIR
ULTRAVIOLET RADIATION