Restoration of X-ray resistance and V(D)J recombination in mutant cells by Ku cDNA
- Stanford Univ. Medical Center, CA (United States)
- Washington Univ. School of Medicine, St. Louis, MO (United States)
Three genetic complementation groups of rodent cells are defective for both repair of x-ray-induced double-strand breaks and V(D)J recombination. Cells from one group lack a DNA end-binding activity that is biochemically and antigenically similar to the Ku autoantigen. Transfection of complementary DNA (cDNA) that encoded the 86-kilodalton subunit of Ku rescued these mutant cells for DNA end-binding activity, x-ray resistance, and V(D)J recombination activity. These results establish a role for Ku in DNA repair and recombination. Furthermore, as a component of a DNA-dependent protein kinase, Ku may initiate a signaling pathway induced by DNA damage.
- Sponsoring Organization:
- USDOE
- OSTI ID:
- 70337
- Journal Information:
- Science, Journal Name: Science Journal Issue: 5183 Vol. 266; ISSN SCIEAS; ISSN 0036-8075
- Country of Publication:
- United States
- Language:
- English
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