Monoclonal antibodies recognizing L-thyroxine

Patent · Tue Jan 13 04:00:00 EST 1987

OSTI ID:7033220

Siebert, G R; Armstrong, J

A method is described for the radioimmunoassay of thyroxine comprising: (a) affixing to a solid phase particular mouse monoclonal antibodies produced by hybridoma cell lines designated as ATCC HB 8499 and ATCC HB 8500, the antibodies being specific for thyroxine and having a cross reactivity to triiodothyronine of no more than 0.12%; (b) reacting the monoclonal antibodies with a composition comprising thyroxine and a predetermined amount of radiolabeled thyroxine to cause binding therebetween; (c) separating the solid phase from unreacted thyroxine and radiolabeled thyroxine; (d) determining the amount of radiolabeled thyroxine bound on the solid phase; (e) repeating steps (a) to (d) with other compositions comprising predetermined and different amounts of thyroxine; (f) repeating steps (a) to (d) with a composition comprising an unknown amount of thyroxine; and (g) determining the unknown amount of thyroxine by ascertaining which of the solid phases reacted with a predetermined amount of thyroxine has the same amount of bound radiolabeled thyroxine as the solid phase reacted with the unknown amount of thyroxine.

• Assignee: Becton, Dickinson and Co., Franklin Lakes, NJ
• Patent Number(s): US 4636478
• OSTI ID: 7033220
• Country of Publication: United States
• Language: English

Related Subjects

550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
AMINO ACIDS
ANIMAL CELLS
ANIMALS
ANTIBODIES
CARBOXYLIC ACIDS
DESIGN
HORMONES
HYBRIDOMAS
IMMUNOASSAY
IMMUNOLOGY
ISOTOPE APPLICATIONS
LABELLING
MAMMALS
MICE
MONOCLONAL ANTIBODIES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
ORGANIC IODINE COMPOUNDS
PEPTIDE HORMONES
QUANTITY RATIO
RADIOASSAY
RADIOIMMUNOASSAY
RADIOIMMUNOLOGY
RODENTS
SPECIFICITY
THYROID HORMONES
THYROXINE
TRACER TECHNIQUES
TRIIODOTHYRONINE
VERTEBRATES