Ionic strength and temperature induced conformational changes in mononucleosomes and oligonucleosomes. [Chromatin]

Journal Article · Wed Oct 01 00:00:00 EDT 1980 · Biophys. J.; (United States)

DOI:https://doi.org/10.1016/S0006-3495(80)84950-2· OSTI ID:7032725

Schmitz, K S; Kent, J C; Parthasarathy, N; Radhakrishnan, G; Ramanathan, B

Chromatin is a nucleohistone complex which exhibits a repeat unit structure as inferred from nuclease digestion studies. The repeat unit, or nucleosome, is defined as approx. 200 base pairs of DNA wrapped about the surface of an octameric histone complex (two copies each of the histones H2A, H2B, H3, and H4). We report in this communication preliminary studies on the conformation of chromatin mononucleosomes and oligonucleosomes as a function of temperature and ionic strength. The methods used were conductivity, fluorescence of bound proflavine, and quasielastic light scattering.

• Research Organization: Univ. of Missouri, Kansas City
• OSTI ID: 7032725
• Journal Information: Biophys. J.; (United States), Vol. 32:1, Issue 1
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
CHROMATIN
STRUCTURAL CHEMICAL ANALYSIS
HEAT
SPATIAL DEPENDENCE
IONIC COMPOSITION
CONFIGURATION INTERACTION
HISTONES
MOLECULAR STRUCTURE
ENERGY
ORGANIC COMPOUNDS
PROTEINS
550200* - Biochemistry