Estimation of vitamin D/sub 2/ vitamin D/sub 3/, and their 25-hydroxy metabolites in animal tissues and foods

Thesis/Dissertation · Thu Jan 01 00:00:00 EST 1987

OSTI ID:7031417

Travis, B D

A method was developed for the determination of vitamins D/sub 2/ and D/sub 3/ and their 25-hydroxy metabolites that could be applicable to a variety of tissues and foods. Tritiated vitamin D/sub 3/ and 25-hydroxyvitamin D/sub 3/ were used to monitor vitamin losses through the assay procedure. The first step used a saponification and extraction to free the vitamins D from their matrix and reduce the lipids. Most samples were saponified using the AOAC method, while a new procedure was developed for high fat samples. This utilized greater extraction volumes, a more polar extraction solvent, and three salt washes. Three different types of chromatography were then used to enable quantitation. The method was reproducible and accurate for the estimation of vitamin D/sub 3/ and 25-hydroxyvitamin D/sub 3/, but not as accurate for determining vitamin D/sub 2/ and 25-hydroxyvitamin D/sub 2/ due to a lack of radioisotope standards of these compounds.

• Research Organization: Illinois Univ., Urbana (USA)
• OSTI ID: 7031417
• Resource Relation: Other Information: Thesis (Ph. D.)
• Country of Publication: United States
• Language: English

Related Subjects

37 INORGANIC
ORGANIC
PHYSICAL AND ANALYTICAL CHEMISTRY
CHOLECALCIFEROL
QUANTITATIVE CHEMICAL ANALYSIS
ERGOCALCIFEROL
METABOLITES
ANIMAL TISSUES
CHROMATOGRAPHY
FOOD
HYDROXY COMPOUNDS
SAPONIFICATION
TRACER TECHNIQUES
TRITIUM COMPOUNDS
BODY
CHEMICAL ANALYSIS
CHEMICAL REACTIONS
DECOMPOSITION
HYDROLYSIS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LYSIS
ORGANIC COMPOUNDS
SEPARATION PROCESSES
SOLVOLYSIS
TISSUES
VITAMIN D
VITAMINS
400101* - Activation
Nuclear Reaction
Radiometric & Radiochemical Procedures