Assignment of xeroderma pigmentosum group C(XPC) gene to chromosome 3p25

Journal Article · Sun May 01 04:00:00 EDT 1994 · Genomics; (United States)

DOI:https://doi.org/10.1006/geno.1994.1256· OSTI ID:7029790

Legerski, R J; Liu, P; Li, L; Peterson, C A; Zhao, Y; Siciliano, M J ^[1]; Leach, R J; Naylor, S L ^[2]

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1. Univ. of Texas M.D. Anderson Cancer Center, Houston, TX (United States)
2. Univ. of Texas Health Science Center, San Antonio, TX (United States)

The human gene XPC (formerly designated XPCC), which corrects the repair deficiency of xeroderma pigmentosum (XP) group C cells, was mapped to 3p25. A cDNA probe for Southern blot hybridization and diagnostic PCR analyses of hybrid clone panels informative for human chromosomes in general and portions of chromosome 3 in particular produced the initial results. Fluorescence in situ hybridization utilizing both a yeast artificial chromosome DNA containing the gene and XPC cDNA as probes provided verification and specific regional assignment. A conflicting assignment of XPC to chromosome 5 is discussed in light of inadequacies in the exclusive use of microcell-mediated chromosome transfer for gene mapping. 12 refs., 3 figs.

• OSTI ID: 7029790
• Journal Information: Genomics; (United States), Journal Name: Genomics; (United States) Vol. 21:1; ISSN 0888-7543; ISSN GNMCEP
• Country of Publication: United States
• Language: English

Related Subjects

550400* -- Genetics
59 BASIC BIOLOGICAL SCIENCES
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
BIOTECHNOLOGY
CHROMOSOMES
CONGENITAL DISEASES
DISEASES
DNA HYBRIDIZATION
DNA REPAIR
ETIOLOGY
GENE AMPLIFICATION
GENETIC ENGINEERING
GENETIC MAPPING
HEREDITARY DISEASES
HUMAN CHROMOSOME 3
HUMAN CHROMOSOMES
HYBRIDIZATION
MAPPING
NUCLEIC ACID HYBRIDIZATION
POLYMERASE CHAIN REACTION
REPAIR
SKIN DISEASES
XERODERMA PIGMENTOSUM