Quantitation of apoB-48 and apoB-100 by gel scanning or radioiodination
- Cornell Univ., Ithaca, NY (USA)
In this presentation, we have validated two procedures for the separation and quantitation of apoB-48 and apoB-100 by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE): (1) gamma counting of radioiodinated lipoproteins and (2) scanning of stained gels. Total apoB in SDS solutions was determined by absorbance at 220 nm, and validated by amino acid analysis. The absorbance at 220 nm, in contrast to the Lowry procedure, could be used with BSA as a standard without correction factors. At relative apoB-48 concentrations higher than 10% of total apoB, both scanning and radioiodination gave reliable results. At lower relative apoB-48 concentrations, the radioiodine method appeared to be superior, but at low total apoB concentrations, the efficiency of radioiodination was low.
- OSTI ID:
- 7027425
- Journal Information:
- Journal of Lipid Research; (USA), Journal Name: Journal of Lipid Research; (USA) Vol. 30:10; ISSN JLPRA; ISSN 0022-2275
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
AMINO ACIDS
ANIMALS
APOLIPOPROTEINS
CARBOXYLIC ACIDS
CHEMICAL REACTIONS
COUNTING TECHNIQUES
ELECTROPHORESIS
HALOGENATION
IODINATION
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LIPIDS
LIPOPROTEINS
MAMMALS
ORGANIC ACIDS
ORGANIC COMPOUNDS
PROTEINS
RABBITS
RATS
RODENTS
SCINTILLATION COUNTING
TESTING
TRACER TECHNIQUES
VALIDATION
VERTEBRATES