Time-resolved resonance Raman spectroscopy of bacteriorhodopsin on the millisecond timescale

Journal Article · Thu Dec 01 04:00:00 EST 1977 · Proc. Natl. Acad. Sci. U.S.A.; (United States)

DOI:https://doi.org/10.1073/pnas.74.12.5212· OSTI ID:7027345

Terner, J; Campion, A; El-Sayed, M A

A simple technique is described that uses a continuous wave laser with electromechanical modulation to obtain time-resolved Raman spectra of transient species on the millisecond timescale. The time behavior of the vibrational bands of the intermediates involved in the proton pumping of bacteriorhodopsin is determined. From these results, along with resonance enhancement and power dependence studies, the bands that appear in the continuous wave Raman spectrum of bacteriorhodopsin can be assigned to three intermediates in the photochemical cycle of bacteriorhodopsin, bR/sub 570/, bL/sub 550/, and bM/sub 412/. The Raman spectra of bR/sub 570/ and bM/sub 412/ are compared with published spectra of model Schiff bases of all-trans and 13-cis retinal.

• Research Organization: Univ. of California, Los Angeles
• OSTI ID: 7027345
• Journal Information: Proc. Natl. Acad. Sci. U.S.A.; (United States), Journal Name: Proc. Natl. Acad. Sci. U.S.A.; (United States) Vol. 74:12; ISSN PNASA
• Country of Publication: United States
• Language: English

Related Subjects

550200* -- Biochemistry
550700 -- Microbiology
59 BASIC BIOLOGICAL SCIENCES
BACTERIA
BIOCHEMICAL REACTION KINETICS
CHEMICAL REACTIONS
CHEMISTRY
KINETICS
LASERS
MICROORGANISMS
PHOTOCHEMICAL REACTIONS
PHOTOCHEMISTRY
PHOTOSYNTHESIS
PIGMENTS
RAMAN SPECTRA
REACTION KINETICS
RESOLUTION
SPECTRA
SYNTHESIS
TIME RESOLUTION
TIMING PROPERTIES