Effects of anion transport inhibitors and ion substitution on Cl sup minus transport in TAL of Henle's loop

Journal Article · Tue Dec 01 00:00:00 EST 1987 · American Journal of Physiology; (USA)

OSTI ID:7027254

Kondo, Yoshiaki; Yoshitomi, Koji; Imai, Masashi ^[1]

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1. National Cardiovascular Center Research Institute, Osaka (Japan)

To identify the mechanism of Cl{sup {minus}} transport across the thin ascending limb of Henle's loop (TAL), the authors examined effects of anion transport inhibitors and ionic substitution in the isolated segments of hamsters using the in vitro microperfusion technique. 4,4{prime}-diisothiocyanostilbene-2,2{prime}-disulfonic acid (DIDS) at 10{sup {minus}3} M changed the NaCl diffusion voltage (V{sub t}) to the orientation that corresponds to the decrease in the Cl{sup {minus}}-Na{sup +} permeability ratio when it was added either to the bath or to the lumen. DIDS, added to the bath or to the lumen decreased the lumen-to-bath flux coefficient for {sup 36}Cl, whereas it had little effect on the flux coefficient for {sup 22}Na. The inhibitory effect of phloretin was rapid and reversible. Phloridzin was ineffective. From these observations, they conclude that Cl{sup {minus}} transport across the TAL is distinct from Na{sup +} and is not coupled with Na{sup +}, K{sup +}, or HCO{sup {minus}}{sub 3}.

• OSTI ID: 7027254
• Journal Information: American Journal of Physiology; (USA), Vol. 253:6; ISSN 0002-9513
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
CHLORINE 36
MEMBRANE TRANSPORT
ENZYME INHIBITORS
BIOCHEMISTRY
RENAL CLEARANCE
BIOCHEMICAL REACTION KINETICS
SODIUM 22
ANIONS
DOSE-RESPONSE RELATIONSHIPS
HAMSTERS
KIDNEYS
MEMBRANE PROTEINS
OSMOSIS
POTASSIUM CHLORIDES
SODIUM CHLORIDES
SULFONIC ACIDS
ALKALI METAL COMPOUNDS
ALKALI METAL ISOTOPES
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BETA-PLUS DECAY RADIOISOTOPES
BODY
CHARGED PARTICLES
CHEMISTRY
CHLORIDES
CHLORINE COMPOUNDS
CHLORINE ISOTOPES
CLEARANCE
DIFFUSION
ELECTRON CAPTURE RADIOISOTOPES
EXCRETION
HALIDES
HALOGEN COMPOUNDS
IONS
ISOMERIC TRANSITION ISOTOPES
ISOTOPES
KINETICS
LIGHT NUCLEI
MAMMALS
NUCLEI
ODD-ODD NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
ORGANS
POTASSIUM COMPOUNDS
PROTEINS
RADIOISOTOPES
REACTION KINETICS
RODENTS
SODIUM COMPOUNDS
SODIUM ISOTOPES
VERTEBRATES
YEARS LIVING RADIOISOTOPES
550201* - Biochemistry- Tracer Techniques