Whole body glucose kinetics in type I diabetes studied with (6,6-/sup 2/H) and (U-/sup 13/C)-glucose and the artificial B-cell

Journal Article · Sun May 01 04:00:00 EDT 1988 · Metab., Clin. Exp.; (United States)

DOI:https://doi.org/10.1016/0026-0495(88)90052-2· OSTI ID:7025606

Darmaun, D; Cirillo, D; Koziet, J; Chauvet, D; Young, V R; Robert, J J

Dynamic aspects of whole body glucose metabolism were assessed in ten young adult insulin-dependent (type I) diabetic men. Using a primed, continuous intravenous infusion of (6,6-/sup 2/H)glucose and (U-/sup 13/C)glucose, endogenous production, tissue uptake, carbon recycling, and oxidation of glucose were measured in the postabsorptive state. These studies were undertaken after blood glucose had been maintained overnight at 5.9 +/- 0.4 mmol/L (n = 10), and on another night at 10.5 +/- 0.4 mmol/L (n = 4) or 15.2 +/- 0.6 mmol/L (n = 6). In the normoglycemic state, endogenous glucose production averaged 2.15 +/- 0.13 mg x kg-1 x min-1. This value, as well as the rate of glucose carbon recycling (0.16 +/- 0.04 mg x kg-1 x min-1) and glucose oxidation (1.52 +/- 0.16 mg x kg-1 x min-1) are comparable to those found in nondiabetic controls. In the hyperglycemic states at 10 or 15 mmol/L, endogenous glucose production was increased by 11% (P less than .01) and 60% (P less than .01) compared to the normoglycemic states, respectively. Glucose carbon recycling contributed only a small percentage to this variation in glucose production (15% at the 15 mmol/L glucose level). This suggests that if gluconeogenesis participates in the increased glucose output, it is not dependent on a greater systemic supply of three-carbon precursors. The increased rate of glucose production in the hyperglycemic state was quantitatively offset by a rise in urinary glucose excretion. Glucose tissue uptake, as well as glucose oxidation, did not vary between normoglycemic and hyperglycemic states.

• Research Organization: Hopital Herold, Paris (France)
• OSTI ID: 7025606
• Journal Information: Metab., Clin. Exp.; (United States), Journal Name: Metab., Clin. Exp.; (United States) Vol. 37:5; ISSN METAA
• Country of Publication: United States
• Language: English

Related Subjects

550901* -- Pathology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALDEHYDES
CARBOHYDRATES
CARBON 13
CARBON ISOTOPES
CHEMICAL REACTIONS
DEUTERIUM
DIABETES MELLITUS
DISEASES
ENDOCRINE DISEASES
EVEN-ODD NUCLEI
GLUCOSE
HEXOSES
HORMONES
HYDROGEN ISOTOPES
INSULIN
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
METABOLIC DISEASES
METABOLISM
MONOSACCHARIDES
NUCLEI
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
PATHOGENESIS
PEPTIDE HORMONES
REDOX REACTIONS
SACCHARIDES
STABLE ISOTOPES
TRACER TECHNIQUES