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Differentiation of the insulin-sensitive glucose transporter in 3T3-L1 adipocytes

Journal Article · · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)
OSTI ID:7025339
; ; ; ;
3T3-L1 fibroblasts differentiate in culture to resemble adipocytes both morphologically and biochemically. Insulin-sensitive glucose transport, as measured by 2-deoxy-(1-/sup 14/C)- glucose uptake in the undifferentiated cell is small (2X). In contrast, the rate of glucose transport in fully differentiated cells is elevated 15-fold over basal in the presence of insulin. To determine if this is due to an increase in the number of transporters/cell or accessibility to the transporters, the number of transporters was measured in subcellular fractions over differentiation using a /sup 3/H-cytochalasin B binding assay. The increase in the rate of insulin-sensitive glucose transport directly parallels an increase in the number of transporters which reside in an insulin-responsive intracellular compartment. This observation was confirmed by identifying the transporters by immunoblotting using an antibody generated against the human erythrocyte transporter. The molecular weight of this transporter increases over differentiation from a single band of 40kDa to a heterogeneous triplet of 40, 44 and 48kDa. These data suggest that the transporter undergoes differential processing and that the functional, insulin-responsive transporter may be different from the insulin-insensitive (basal) transporter.
Research Organization:
Univ. of Florida School of Medicine, Gainesville
OSTI ID:
7025339
Report Number(s):
CONF-8606151-
Journal Information:
Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Vol. 45:6; ISSN FEPRA
Country of Publication:
United States
Language:
English

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Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ADIPOSE TISSUE
ALDEHYDES
ANIMAL CELLS
ANIMAL TISSUES
ANTIBODIES
BIOASSAY
BIOCHEMICAL REACTION KINETICS
BODY
CARBOHYDRATES
CARBON 14 COMPOUNDS
CELL DIFFERENTIATION
CONNECTIVE TISSUE
CONNECTIVE TISSUE CELLS
FIBROBLASTS
GLUCOSE
HEXOSES
HORMONES
INSULIN
ISOTOPE APPLICATIONS
KINETICS
LABELLED COMPOUNDS
MEMBRANE TRANSPORT
MONOSACCHARIDES
ORGANIC COMPOUNDS
PEPTIDE HORMONES
PROTEINS
REACTION KINETICS
SACCHARIDES
SOMATIC CELLS
TISSUES
TRACER TECHNIQUES
TRITIUM COMPOUNDS