Plasminogen activator activity during growth and differentiation of human HL-60 promyelocytes

Journal Article · Thu May 01 04:00:00 EDT 1986 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:7025216

Kumar, S; Russo, M S

The involvement of plasminogen activators (PA) of urokinase or tissue type which activate plasminogen to plasmin have been implicated in fibrinolysis, differentiation and cancer growth. In the present study, alteration of PA activity of human HL-60 promyelocytes during growth and differentiation has been investigated. HL-60 promyelocytes seeded at a density of 1 x 10/sup 5/ cells/ml, were examined at different densities of growth (0.2-2.0 x 10/sup 6/ cells/ml for PA activity, measured as plasminogen dependent hydrolysis of /sup 125/I labelled fibrin or D-Val-L-Leu-L-Lys-pNA. /sup 125/I-fibrin is hydrolyzed at a linear rate between 2-6 hours and at cell concentration of .2-1 x 10/sup 6/ per ml. The cellular PA activity per 10/sup 6/ cells at different growth densities decreases by 50% between .2-2 x 10/sup 6/ cells/ml. Similarly, when cells are differentiated to monocytes-macrophages by 1,25-dihydroxyvitamin D/sub 3/ (as determined by NBT reduction), a decrease in PA activity is observed which parallels the degree of differentiation. The major alteration of PA activity is in the heavy membrane fraction of the differentiated cells. These studies suggest that PA levels may be important in the growth and differentiation of HL-60 cells.

• Research Organization: New Jersey Medical School, Newark
• OSTI ID: 7025216
• Report Number(s): CONF-8606151-
• Journal Information: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Vol. 45:6; ISSN FEPRA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANIMALS
BETA DECAY RADIOISOTOPES
CELL DIFFERENTIATION
CHEMICAL REACTIONS
COAGULANTS
CONNECTIVE TISSUE CELLS
DAYS LIVING RADIOISOTOPES
DECOMPOSITION
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
ENZYME ACTIVITY
FIBRIN
FIBRINOLYTIC AGENTS
HEMATOLOGIC AGENTS
HEMOSTATICS
HYDROLYSIS
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LYSIS
MACROPHAGES
MAMMALS
MAN
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
PHAGOCYTES
PLASMINOGEN
PRIMATES
PROTEINS
RADIOISOTOPES
SCLEROPROTEINS
SECRETION
SOLVOLYSIS
SOMATIC CELLS
TRACER TECHNIQUES
VERTEBRATES
VITAMIN D
VITAMINS