Deficits in T helper cells after total lymphoid irradiation (TLI): Reduced IL-2 secretion and normal IL-2 receptor expression in the mixed leukocyte reaction (MLR)
- Stanford Univ. School of Medicine, CA (USA)
Spleen cells from BALB/c mice treated with total lymphoid irradiation (TLI) and from normal, unirradiated mice were compared in the mixed leukocyte reaction (MLR). Although the percentage of CD4+ cells in the spleen was close to normal, 4 to 6 weeks after TLI, the MLR of unfractionated spleen cells from irradiated mice was more than 10-fold lower than controls. A similar reduction was observed when purified CD4+ cells were used as responders in the MLR. Secretion of IL-2 by cells from irradiated mice was also about 10-fold lower than controls. However, the percentage of CD4+ and CD8+ cells which expressed IL-2 surface receptors during the MLR was similar using spleen cells from irradiated and control mice. Addition of an exogenous source of IL-2 restored the proliferative capacity of the irradiated cells and suggests that the lack of IL-2 secretion is the likely explanation of the marked deficit in the MLR of CD4+ spleen cells after TLI.
- OSTI ID:
- 7025147
- Journal Information:
- Cellular Immunology; (USA), Journal Name: Cellular Immunology; (USA) Vol. 126:1; ISSN 0008-8749; ISSN CLIMB
- Country of Publication:
- United States
- Language:
- English
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63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
ANTIGENS
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BIOLOGICAL RADIATION EFFECTS
BLOOD
BLOOD CELLS
BODY FLUIDS
CELL PROLIFERATION
GROWTH FACTORS
LEUKOCYTES
LYMPHATIC SYSTEM
LYMPHOKINES
MAMMALS
MATERIALS
MEMBRANE PROTEINS
MICE
MITOGENS
ORGANIC COMPOUNDS
PROTEINS
RADIATION EFFECTS
RECEPTORS
RODENTS
SOMATIC CELLS
SPLEEN CELLS
VERTEBRATES