Nonenzymatic glycation of phosphatidylethanolamine in erythrocyte vesicles
Journal Article
·
· Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)
OSTI ID:7024401
Unsealed inside-out and right-side out vesicles were prepared from human red cells. The vesicles were incubated with D-glucose (/sup 14/C(U)) and sodium cyanoborohydride in phosphate buffer, pH 7.4. After incubation, lipids were extracted with 1-butanol and non-lipid contaminants removed by Sephadex G-25 chromatography. Phosphatidylethanolamine-sorbitol was purified by chromatography on columns of silicic acid and phenylboronate agarose gel. Phospholipase C (B. cereus) liberated phosphoethanolamine-sorbitol (I) which comigrated on TLC with synthetic I prepared by reductive condensation of phosphoethanolamine and D-glucose and also with the product of phospholipase C (B. cereus) hydrolysis of reference phosphatidylethanolamine-sorbitol. Exposure of I to alkaline phosphatase (E. coli) gave P/sub i/ and ethanolamine-sorbitol (II) which comigrated on TLC with synthetic II prepared by reductive condensation of ethanolamine and D-glucose or by phospholipase D hydrolysis of reference phosphatidylethanolamine-sorbitol. These studies demonstrate that vesicular phosphatidylethanolamine can be reductively glycated and illustrate the applicability of both phospholipase C and phospholipase D in characterizing glycated phosphoglycerides.
- Research Organization:
- New York Medical College, Valhalla
- OSTI ID:
- 7024401
- Report Number(s):
- CONF-8606151-
- Journal Information:
- Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Vol. 45:6; ISSN FEPRA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550501* -- Metabolism-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALDEHYDES
ALKALINE PHOSPHATASE
ANIMALS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CARBOHYDRATES
CARBON 14 COMPOUNDS
CARBOXYLESTERASES
CHROMATOGRAPHY
ENZYMES
ERYTHROCYTES
ESTERASES
ESTERS
GLUCOSE
HEXOSES
HYDROLASES
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LIPASE
LIPIDS
MAMMALS
MAN
MATERIALS
METABOLISM
MONOSACCHARIDES
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
PHOSPHATASES
PHOSPHOLIPIDS
PRIMATES
SACCHARIDES
SEPARATION PROCESSES
TRACER TECHNIQUES
VERTEBRATES
59 BASIC BIOLOGICAL SCIENCES
ALDEHYDES
ALKALINE PHOSPHATASE
ANIMALS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CARBOHYDRATES
CARBON 14 COMPOUNDS
CARBOXYLESTERASES
CHROMATOGRAPHY
ENZYMES
ERYTHROCYTES
ESTERASES
ESTERS
GLUCOSE
HEXOSES
HYDROLASES
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LIPASE
LIPIDS
MAMMALS
MAN
MATERIALS
METABOLISM
MONOSACCHARIDES
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
PHOSPHATASES
PHOSPHOLIPIDS
PRIMATES
SACCHARIDES
SEPARATION PROCESSES
TRACER TECHNIQUES
VERTEBRATES