Inactivation of human osteosarcoma cells in vitro by {sup 211}At-TP-3 monoclonal antibody: Comparison with astatine-211 and external-beam X rays

Journal Article · Mon Aug 01 04:00:00 EDT 1994 · Radiation Research

DOI:https://doi.org/10.2307/3578662· OSTI ID:70232

Larsen, R H ^[1]; Bruland, O S ^[2]; Hoff, P; Alstad, J ^[1]; Lindmo, T ^[2]; Rofstad, E K ^[3]

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1. Univ. of Oslo (Norway)
2. Institute for Cancer Research, Oslo (Norway)
3. Norwegian Institute of Technology, Trondheim (Norway)

The potential usefulness of {alpha}-particle radioimmunotherapy in the treatment of osteosarcoma was studied in vitro by using the monoclonal antibody TP-3 and cells of three human osteosarcoma cell lines (OHS, SAOS and KPDX) differing in antigen expression. Cell survival curves were established after treatment with (a) {sup 211}At-TP-3 of different specific activities, (b) {sup 211}At-labeled bovine serum albumin (BSA), (c) free {sup 211}At and (d) external-beam X rays. The three osteosarcoma cell lines showed similar survival curves, whether treated with external-beam X rays, {sup 211}At-BSA or free {sup 211}At. The D{sub o}`s were lower for free {sup 211}At than for {sup 211}At-BSA. The survival curves for {sup 211}At-TP-3 treatment, on the other hand, differed significantly among the cell lines, suggesting that sensitivity to {sup 211}At-TP-3 treatment was governed by cellular properties other than sensitivity to external-beam X rays. The cellular property most important for sensitivity to {sup 211}At-TP-3 treatment was the antigen expression. Cell inactivation after {sup 211}At-TP-3 treatment increased substantially with increasing specific activity of the {sup 211}At-TP-3. At high specific activities, the cytotoxic effect of {sup 211}At-TP-3 was significantly higher than that of {sup 211}At-BSA. In conclusion, {sup 211}At-TP-3 has the potential to give clinically favorable therapeutic ratios in the treatment of osteosarcoma. 39 refs., 5 figs., 2 tabs.

• Sponsoring Organization: USDOE
• OSTI ID: 70232
• Journal Information: Radiation Research, Journal Name: Radiation Research Journal Issue: 2 Vol. 139; ISSN RAREAE; ISSN 0033-7587
• Country of Publication: United States
• Language: English

Related Subjects

55 BIOLOGY AND MEDICINE
BASIC STUDIES
56 BIOLOGY AND MEDICINE
APPLIED STUDIES
ANTIGEN-ANTIBODY REACTIONS
ASTATINE 211
CELL CULTURES
COMPARATIVE EVALUATIONS
INACTIVATION
MONOCLONAL ANTIBODIES
OSTEOSARCOMAS
RADIOIMMUNOTHERAPY
SENSITIVITY
SURVIVAL CURVES
X RADIATION