Neurotransmitter agonists inhibit inositol phosphate formation in the brain of bupropione-treated rats

Conference · Wed Mar 05 00:00:00 EST 1986 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:7022181

Butler, P D; Hungund, B; Suckow, R; Barkai, A I

Bupropione is a chemically unique antidepressant whose mechanism of action is not known. In this study they have evaluated the effect of chronic treatment with bupropione on the receptor-mediated release of inositol phosphates (IP) from brain slices in rats. Animals were implanted with Alzet osmotic pumps that delivered bupropione at a constant rate (40mg/kg/day) for 2 weeks. Cross-chopped slices of cerebral cortex from control and drug-treated rats were prelabelled with myo-/sup 3/H-inositol in HEPES buffer containing 11 mM LiCl. Accumulation of IP was measured in the presence and absence of the following agonists: Carbamylcholine (100..mu..m); norepinephrine (5..mu..M) and serotonin (10..mu..M). All agonists stimulated release of IP from slices of control animals but appeared to inhibit IP release in bupropione-treated rats. These results indicate that a phospholipase C inhibitor may appear following the activation of this enzyme by the agonist, and that the agonist-induced formation of the apparent inhibitor may be markedly enhanced after treatment with bupropione.

• Research Organization: Columbia Univ. Medical Center, New York, NY
• OSTI ID: 7022181
• Report Number(s): CONF-8604222-
• Journal Information: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Vol. 45:4; Conference: 70. annual meeting of the Federation of American Society for Experimental Biology, St. Louis, MO, USA, 13 Apr 1986
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
59 BASIC BIOLOGICAL SCIENCES
ANTIDEPRESSANTS
BIOLOGICAL EFFECTS
PHOSPHORIC ACID ESTERS
BIOSYNTHESIS
BRAIN
CHOLINE
ENZYME INHIBITORS
INOSITOL
LIPASE
NORADRENALINE
RATS
RECEPTORS
RESPONSE MODIFYING FACTORS
SEROTONIN
TRACER TECHNIQUES
TRITIUM COMPOUNDS
ADRENAL HORMONES
ALCOHOLS
AMINES
AMMONIUM COMPOUNDS
ANIMALS
AROMATICS
AUTONOMIC NERVOUS SYSTEM AGENTS
AZAARENES
AZOLES
BODY
CARBOHYDRATES
CARBOXYLESTERASES
CARDIOTONICS
CARDIOVASCULAR AGENTS
CENTRAL NERVOUS SYSTEM
CENTRAL NERVOUS SYSTEM AGENTS
DRUGS
ENZYMES
ESTERASES
ESTERS
HETEROCYCLIC COMPOUNDS
HORMONES
HYDROLASES
HYDROXY COMPOUNDS
INDOLES
INOSITOLS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LIPOTROPIC FACTORS
MAMMALS
MEMBRANE PROTEINS
MONOSACCHARIDES
NERVOUS SYSTEM
NEUROREGULATORS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
ORGANS
PROTEINS
PSYCHOTROPIC DRUGS
PYRROLES
QUATERNARY COMPOUNDS
RADIOPROTECTIVE SUBSTANCES
RODENTS
SACCHARIDES
STEROID HORMONES
SYMPATHOMIMETICS
SYNTHESIS
TRYPTAMINES
VERTEBRATES
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