Lesion measurement in non-radioactive DNA by quantitative gel electrophoresis

Conference · Sun Jan 01 04:00:00 EST 1989

OSTI ID:7018990

Sutherland, J C; Chen, Chun Zhang; Emrick, A; Hacham, H; Monteleone, D; Ribeiro, E; Trunk, J; Sutherland, B M

The gel electrophoresis method developed during the past ten years in our laboratories makes possible the quantitation of UV induced pyrimidine dimers, gamma ray induced single- and double-strand breaks and many other types of lesions in nanogram quantities of DNA. The DNA does not have to be labeled with radionuclides or of a particular conformation, thus facilitating the use of the method in measuring damage levels and repair rates in the DNA of intact organisms -- including man. The gel method can quantitate any lesion in DNA that either is, or can be converted to a single- or double-strand break. The formation of a strand break produces two shorter DNA molecules for each molecule that existed before the treatment that produced the break. Determining the number of breaks, and hence the number of lesions, becomes a matter of comparing the average lengths of molecules in samples differing only in lesion-induced breaks. This requires that we determine the distribution of mass of DNA on a gel as a function of its distance of migration and also the dispersion function of its distance of migration and also the dispersion function (the relationship between molecular length and distance of migration) in the gel electrophoresis system. 40 refs., 5 figs.

• Research Organization: Brookhaven National Lab., Upton, NY (USA)
• Sponsoring Organization: DOE/ER
• DOE Contract Number: AC02-76CH00016
• OSTI ID: 7018990
• Report Number(s): BNL-43231-1; BIO--4558; CONF-8910354--1; ON: DE90007891
• Country of Publication: United States
• Language: English

Related Subjects

550200* -- Biochemistry
560120 -- Radiation Effects on Biochemicals
Cells
& Tissue Culture
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
CHEMICAL ANALYSIS
COLLOIDS
DISPERSIONS
DNA
DNA SEQUENCING
DOSE-RESPONSE RELATIONSHIPS
ELECTROMAGNETIC RADIATION
ELECTROPHORESIS
EXTREME ULTRAVIOLET RADIATION
GELS
MOLECULAR STRUCTURE
NUCLEIC ACIDS
OPTIMIZATION
ORGANIC COMPOUNDS
PYRIMIDINE DIMERS
QUANTITATIVE CHEMICAL ANALYSIS
RADIATIONS
RADIOINDUCTION
SEPARATION PROCESSES
STRUCTURAL CHEMICAL ANALYSIS
ULTRAVIOLET RADIATION