Decreased triiodothyronine receptor binding in skeletal muscle nuclei and erythrocyte membranes of obese (ob/ob) mice

Thesis/Dissertation · Fri Jan 01 04:00:00 EST 1988

OSTI ID:7018866

Gilvary, E P

Hindlimb skeletal muscle weights and binding of L-tri-iodothyronine (T{sub 3}) to isolated nuclei of this tissue were investigated in obese (ob/ob) mice and their lean littermates. Maximal binding capacities (Bmax) and dissociation constants (Kd) were determined by incubating isolated muscle nuclei with increasing conc. of {sup 125}I-T{sub 3} (0.4 nM to 4nM). At 12 wks. of age, although weighing substantially more, obese mice had only 55% as much muscle mass as their lean littermates. There was no phenotype effect observed for Kd, however, Bmax was significantly less for the obese mice. In a second experiment, a 16-wk. feeding study was conducted with 4 groups of mice according to the following design: lean mice fed rodent chow; obese mice fed rodent chow; obese mice, n-6 fatty acid (FA)-rich diet; and obese mice, n-3FA-rich diet. Erythrocyte T{sub 3} receptor binding capacities were measured by incubating red cell ghosts from mice of these 4 groups with {sup 125}I-T{sub 3}. As with skeletal muscle nuclei there were no phenotype effects observed for Kd between any two groups. In contrasts obese mice fed chow and n-6FA-rich diets both exhibited lower Bmax than their lean counterparts, while no significant difference was observed between the latter group and the obese mice fed an n-3FA-rich diet. Bmax values of the n-6 group were also decreased compared to the n-3 group.

• Research Organization: Rutgers--the State Univ., New Brunswick, NJ (USA)
• OSTI ID: 7018866
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ADIPOSE TISSUE
ANIMAL TISSUES
ANIMALS
BETA DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY
BODY FLUIDS
CELL CONSTITUENTS
CELL MEMBRANES
CELL NUCLEI
CONNECTIVE TISSUE
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
ERYTHROCYTES
HORMONES
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
MAMMALS
MATERIALS
MEMBRANE PROTEINS
MEMBRANES
MICE
MUSCLES
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
PEPTIDE HORMONES
PHENOTYPE
PROTEINS
RADIOISOTOPES
REACTION KINETICS
RECEPTORS
RODENTS
THYROID HORMONES
TISSUES
TRACER TECHNIQUES
TRIIODOTHYRONINE
VERTEBRATES