O3-induced airway hyperresponsiveness to noncholinergic system and other stimuli

Journal Article · Wed Jul 01 00:00:00 EDT 1992 · Journal of Applied Physiology (1985); (United States)

OSTI ID:7013378

Campos, M G; Segura, P; Vargas, M H; Vanda, B; Ponce-Monter, H; Selman, M; Montano, L M ^[1]

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1. Departamento de Fisiologia y Farmacologia, Facultad de Medicina Veterinaria y Zootecnia, Universidad Nacional Autonoma de Mexico, DF (Mexico)

The effect of O3 exposure (3 ppm, 1 h) on the in vivo and in vitro airway responsiveness, as well as the changes in cell contents in bronchoalveolar lavage (BAL) fluid, were evaluated 16-18 h after O3 exposure in sensitized and nonsensitized male guinea pigs. The sensitization procedure was performed through repeated inhalation of ovalbumin for 3 wk. Increase in pulmonary insufflation pressure produced by the excitatory nonadrenergic noncholinergic (eNANC) system, histamine, and antigen were assessed in in vivo conditions, whereas airway responsiveness to histamine and substance P was evaluated in in vitro conditions by use of tracheal chains with or without epithelium and lung parenchymal strips. The authors found that O3 exposure (1) increased the neutrophil content in BAL fluids in both sensitized and nonsensitized guinea pigs, (2) caused hyperresponsiveness to eNANC stimulation in nonsensitized guinea pigs (although combination of sensitization and O3 exposure paradoxically abolished the hyperresponsiveness to eNANC stimulation), (3) increased the in vivo bronchoconstrictor responses to histamine and antigen, (4) caused hyperresponsiveness to substance P in nonsensitized tracheae with or without epithelium and in sensitized tracheae with epithelium, (5) did not modify the responsiveness to histamine in tracheae with or without epithelium (and in addition, epithelium removal caused hyperresponsiveness to histamine even in those tracheae exposed to O3), and (6) produced hyperresponsiveness to histamine in lung parenchymal strips either from sensitized or nonsensitized guinea pigs.

• OSTI ID: 7013378
• Journal Information: Journal of Applied Physiology (1985); (United States), Journal Name: Journal of Applied Physiology (1985); (United States) Vol. 73:1; ISSN 8750-7587; ISSN JAPHE
• Country of Publication: United States
• Language: English

Related Subjects

560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AMINES
ANIMAL TISSUES
ANIMALS
AUTONOMIC NERVOUS SYSTEM
AZOLES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY
BODY FLUIDS
CARBOHYDRATES
EPITHELIUM
GLUCOPROTEINS
GLYCOPROTEINS
GUINEA PIGS
HETEROCYCLIC COMPOUNDS
HISTAMINE
IMIDAZOLES
IN VITRO
IN VIVO
INHALATION
INTAKE
KININS
LAVAGE
LEUKOCYTES
MAMMALS
MATERIALS
NERVOUS SYSTEM
NEUTROPHILS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
OVALBUMIN
OZONE
PEPTIDES
POLYPEPTIDES
PROTEINS
RESPIRATORY SYSTEM
RODENTS
SACCHARIDES
SENSITIVITY
TISSUES
TOXICITY
TRACHEA
VERTEBRATES