Reexamination of the role of Lyt-2-positive T cells in murine skin graft rejection
The authors have investigated which T cell subclass defined by cytolysis with monoclonal anti-Lyt-1.2 and anti-Lyt-2.2 antibodies is required to adoptively transfer the ability to reject skin grafts. B6.Thy-1.1 spleen cells immune to graft antigens were fractionated with antibody plus C' and transferred to adult thymectomized, irradiated, bone marrow-reconstituted (ATXBM) B6.Thy-1.2 hosts that were simultaneously grafted with BALB.B skin. The authors found that when the ATXBM hosts were used 6 wk after irradiation and marrow reconstitution, both Lyt-1-depleted and Lyt-2-depleted immune spleen cells could transfer the ability to promptly reject skin grafts. However, such ATXBM recipients of Lyt-2-depleted cells that had rejected skin grafts were found to contain graft-specific CTL that were largely of host (B6.Thy-1.2) origin. When ATXBM hosts were used for the experiment 1 wk after irradiation and marrow reconstitution, no host-derived graft-specific CTL could be detected. However, graft rejection occurred in recipients of anti-Lyt-1- or anti-Lyt-2 plus C'-treated immune cells and specific CTL were generated from spleen cells of both groups. Thus, in the absence of a host-derived response, adoptively transferred immune Lyt-2+ cells, either resistant to, or that escaped from, antibody plus C' treatment, are able to expand in response to the antigenic stimulus provided by the graft. A more complete elimination of specific T cell subclasses is therefore needed to assess the relative contribution of a particular subset to the graft rejection process.
- Research Organization:
- Scripps Clinic and Research Foundation, La Jolla, CA
- OSTI ID:
- 7013144
- Journal Information:
- J. Exp. Med.; (United States), Journal Name: J. Exp. Med.; (United States) Vol. 159:1; ISSN JEMEA
- Country of Publication:
- United States
- Language:
- English
Similar Records
Bone marrow-derived T lymphocytes responsible for allograft rejection
Prolonged minor allograft survival in intravenously primed mice--a test of the veto hypothesis
Related Subjects
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL TISSUES
ANIMALS
ANTIBODIES
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BODY
BONE MARROW
CHIMERAS
GRAFT-HOST REACTION
GRAFTS
HEMATOPOIETIC SYSTEM
IMMUNE REACTIONS
IMMUNOSUPPRESSION
MAMMALS
MICE
MONOCLONAL ANTIBODIES
MOSAICISM
ORGANS
RADIATION CHIMERAS
RADIATION EFFECTS
RODENTS
SKIN
TISSUES
TRANSPLANTS
VERTEBRATES