Mutagenesis and lethality following S phase irradiation of xeroderma pigmentosum and normal human diploid fibroblasts with ultraviolet light

Journal Article · Tue Nov 01 04:00:00 EST 1983 · Carcinogenesis (N.Y.); (United States)

DOI:https://doi.org/10.1093/carcin/4.11.1389· OSTI ID:7012716

Grosovsky, A J; Little, J B

The mutagenic and lethal effects of u.v. light exposure in the DNA synthetic phase of the cell cycle were determined in xeroderma pigmentosum complementation group A (XP-A), hereditary adenomatosis of the colon and rectum (ACR), and a normal, foreskin derived cell strain (AG1522). For AG1522, an increased sensitivity to the cytotoxic effects of u.v. light (survival curve D0 . 3.2 J/m2) was observed as compared to previous findings for confluent, non-proliferating cultures (D0 . 4.2 J/m2). XP-A fibroblasts were markedly hypersensitive (D0 . 0.5 J/m2) and ACR fibroblasts exhibited an intermediate response (D0 . 2.0 J/m2). The mutagenic response of ACR fibroblasts, however, was similar to normal fibroblasts. A threshold of 1.5-2 J/m2 was observed for u.v. induced mutagenesis in normal and ACR fibroblasts. XP fibroblasts, on the other hand, were strikingly hypermutable and demonstrated little or no threshold. When S phase mutagenesis was considered as a function of survival level rather than u.v. light dose, XP fibroblasts remained significantly hypermutable as compared with normal fibroblasts at all survival levels. Previous mutagenesis results with confluent, nonproliferating cultures of XP and normal fibroblasts were reanalyzed as a function of cytotoxicity; XP hypermutability at all survival levels was also observed.

• Research Organization: Harvard Univ., Boston, MA
• OSTI ID: 7012716
• Journal Information: Carcinogenesis (N.Y.); (United States), Journal Name: Carcinogenesis (N.Y.); (United States) Vol. 4:11; ISSN CRNGD
• Country of Publication: United States
• Language: English

Related Subjects

560301* -- Chemicals Metabolism & Toxicology-- Cells-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
BIOLOGICAL EFFECTS
CELL CULTURES
CELL KILLING
CONNECTIVE TISSUE CELLS
DISEASES
DNA
DOSES
ELECTROMAGNETIC RADIATION
FIBROBLASTS
LETHAL DOSES
MUTAGENESIS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PHOTOSENSITIVITY
RADIATIONS
SENSITIVITY
SKIN DISEASES
SOMATIC CELLS
SURVIVAL CURVES
ULTRAVIOLET RADIATION
XERODERMA PIGMENTOSUM