Effect of Heavy metals on the iron oxidizing ability of Thiobacillus ferrooxidans: Part 1, Effect of silver
The effect of silver ions on the iron oxidizing ability of Thiobacillus ferrooxidans was studied using electrochemical and other physics-chemical techniques. Electrochemical investigation was conducted using a method based on redox potential change. Experiments were performed by adding an aliquot of separately prepared concentrate of the bacteria into the solution of ferrous ion and monitoring the redox potential for at least one hour. Pyrite was used as the indicator electrode. Parameters examined were pH, microbial cell density, ferrous, ferric and silver ion concentration, temperature and preconditioning period of the bacteria with silver ions, etc. Results obtained demonstrate that the rate of ferrous ion oxidation is dependent on pH (optimum pH range is 1.5--2.0) and the substrate (i.e. Fe(II)) to microbial cell concentration ratio. The mechanism of the bacteria mediated oxidation of ferrous iron is remarkably sensitive to temperature changes. At the vicinity of the optimum temperature (i.e. 25[degree]C), the reaction is likely to be controlled by the diffusion of Fe (II) ions through the cell wall of the bacteria, whereas below the range 18--25[degree]C, reaction kinetics may be the rate controlling factor. In the presence of 10 mg/L silver, the reaction may be kinetically controlled over the temperature range 5.5--25[degree]C. Inhibition of microbial FE(II) oxidation in the presence of silver may take place via a mixed mechanism in which silver may bind with both the enzyme and the enzyme-substrate complex.
- Research Organization:
- Idaho Univ., Moscow, ID (United States). Dept. of Metallurgical and Mining Engineering
- Sponsoring Organization:
- USDOE; USDOE, Washington, DC (United States)
- DOE Contract Number:
- FG22-88PC88920
- OSTI ID:
- 7010600
- Report Number(s):
- DOE/PC/88920-T24; ON: DE93004939
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
IRON COMPOUNDS
BIODEGRADATION
SILVER
THIOBACILLUS FERROXIDANS
METABOLISM
BIOCHEMICAL REACTION KINETICS
ELECTROCHEMISTRY
MICROBIAL LEACHING
PROGRESS REPORT
PYRITE
BACILLUS
BACTERIA
CHEMICAL REACTIONS
CHEMISTRY
DECOMPOSITION
DISSOLUTION
DOCUMENT TYPES
ELEMENTS
KINETICS
LEACHING
METALS
MICROORGANISMS
MINERALS
REACTION KINETICS
SEPARATION PROCESSES
SULFIDE MINERALS
SULFUR-OXIDIZING BACTERIA
TRANSITION ELEMENT COMPOUNDS
TRANSITION ELEMENTS
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