Physical analysis of the terminal 240 kb of DNA from human chromosome 7q

Journal Article · Thu Jul 01 04:00:00 EDT 1993 · Genomics; (United States)

DOI:https://doi.org/10.1006/geno.1993.1278· OSTI ID:7009619

Riethman, H C; Spais, C ^[1]; Buckingham, J; Grady, D; Moyzis, R K ^[2]

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1. Wistar Institute of Anatomy and Biology, Philadelphia, PA (United States)
2. Los Alamos National Lab., NM (United States)

DNA from a 240-kb human telomeric yeast artificial chromosome (HTY) clone was analyzed using physical mapping methods. Cosmid subclones of the YAC were fingerprinted using restriction enzyme digestion and human repeat sequence hybridization and then assembled into two contigs that together span 93% of the human insert. Data from restriction mapping and Bal31 exonuclease experiments indicate that, except for the truncation of distal genomic (T[sub 2]AG[sub 3])[sub n] sequences, the molecular clone HTY 146 contains a contiguous, 230-kb telomere-terminal fragment from 7qter. Markers derived from this clone will allow telomeric closure of the physical and genetic linkage maps of human chromosome 7q. 44 refs., 6 figs.

• OSTI ID: 7009619
• Journal Information: Genomics; (United States), Journal Name: Genomics; (United States) Vol. 17:1; ISSN 0888-7543; ISSN GNMCEP
• Country of Publication: United States
• Language: English

Related Subjects

550400* -- Genetics
59 BASIC BIOLOGICAL SCIENCES
CHROMOSOMES
CLONING
DNA HYBRIDIZATION
DNA-CLONING
GENETIC MAPPING
HUMAN CHROMOSOME 7
HUMAN CHROMOSOMES
HYBRIDIZATION
MAPPING