Effects of carbon monoxide on cardiac muscle cells in culture

Journal Article · Thu Sep 01 00:00:00 EDT 1988 · American Journal of Physiology; (USA)

OSTI ID:7009527

Nag, A C; Chen, K C; Cheng, Mei ^[1]

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1. Oakland Univ., Rochester, MI (USA) General Motors Research Laboratories, Warren, MI (USA)

Embryonic rat cardiac muscle cells grown in the presence of various tensions of CO (5-95%) without the presence of O{sub 2} survived and exhibited reduced cell growth, which was concentration dependent. When cardiac muscle cells were grown in the presence of a mixture of CO (10-20%) and O{sub 2} (10-20%), the growth rate of these cells was comparable to that of the control cells. Cardiac myocytes continued to beat when exposed to varying tensions of CO, except in the case of 95% CO. The cells exposed to different concentrations of CO contained fewer myofibrils of different stages of differentiation compared with the control and the culture exposed to a mixture of 20% O{sub 2} and 20% CO, with cells that contained abundant, highly differentiated myofibrils. There was no significant difference in the structural organization of mitochondria between the control and the surviving experimental cells. It is evident from the present studies that O{sub 2} is required for the optimum in vitro cellular growth of cardiac muscle. Furthermore, CO in combination with O{sub 2} at a concentration of 10 or 20% can produce optimal growth of cardiac muscle cells in culture. To determine maximum labeling index during the labeling period, cells were continuously labeled with ({sup 3}H)thymidine for 24 h before the termination of cultures.

• OSTI ID: 7009527
• Journal Information: American Journal of Physiology; (USA), Vol. 255:3; ISSN 0002-9513
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
CARBON MONOXIDE
BIOLOGICAL EFFECTS
HEART
TRANSMISSION ELECTRON MICROSCOPY
CELL CULTURES
MITOTIC INDEX
MUSCLES
SOMATIC CELLS
THYMIDINE
TRITIUM COMPOUNDS
ANIMAL CELLS
AZINES
BODY
CARBON COMPOUNDS
CARBON OXIDES
CARDIOVASCULAR SYSTEM
CHALCOGENIDES
ELECTRON MICROSCOPY
HETEROCYCLIC COMPOUNDS
HYDROGEN COMPOUNDS
MICROSCOPY
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
OXIDES
OXYGEN COMPOUNDS
PYRIMIDINES
RIBOSIDES
550301* - Cytology- Tracer Techniques