Identification of a new human V[lambda] gene family-V[lambda]X
- Univ. of Toronto, Toronto Bayview Regional Cancer Centre, Ontario (Canada) Sunnybrook Health Science Centre, Toronto, Ontario (Canada)
Although approximately 40% of the Ig light chains in human sera are of the Ig[Lambda] isotype, until recently very little was known about the human Ig[lambda] gene locus. The genomic structure of the Ig[lambda] C region has been determined and consists of seven tandemly organized J-C[lambda] segments. The structure of the region containing the Ig[lambda] V gene segments is less well defined. Based on amino acid and nucleotide sequence homologies, the V[lambda] gene segments isolated have been grouped into nine families. Here the authors report on the isolation of a V[lambda] gene from the functional rearrangement of a human B lymphoma cell line that has less than 71% nucleotide sequence and 57% deduced amino acid sequence homology to any known V[lambda] gene. According to the current classification schemes, this gene does not belong to any existing V[lambda] family. Thus they suggest that this gene belongs to a new V[lambda] family, V[lambda]X. The genomic counterpart of the rearranged V[lambda] gene, and a second member of the family were isolated. The second member is a pseudogene. Southern analysis suggests that the V[lambda]X family is a small family. The functional V[lambda]X gene has a consensus heptamer and nonamer that differs at two sites from the consensus recombination signal sequence. The promoter region contains a consensus TATA box and an octamer that diverge from the consensus sequence by two base pairs. 26 refs., 5 figs.
- OSTI ID:
- 7009469
- Journal Information:
- Journal of Immunology; (United States), Journal Name: Journal of Immunology; (United States) Vol. 152:10; ISSN 0022-1767; ISSN JOIMA3
- Country of Publication:
- United States
- Language:
- English
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