Gas chromatographic--mass spectrometric quantitation of 16, 16-dimethyl-trans-delta 2-PGE1

Journal Article · Tue Feb 01 00:00:00 EST 1983 · Prostaglandins; (United States)

OSTI ID:7008163

Dimov, V; Green, K; Bygdeman, M; Konishi, Y; Imaki, K; Hayashi, M

Di-deuterated and di-tritiated 16,16-dimethyl-trans-delta 2-PGE1 has been synthesized and used for development of a GC-MS method for quantitation of corresponding unlabelled drug in patient plasma. Although these carrier/internal standard molecules only contain 2 deuterium atoms the lower limit of detection at each injection is as low as about 40 pg. The maximum plasma levels of this drug following administration of vaginal suppositories used in clinical studies (1 mg 16,16-dimethyl-trans-delta 2-PGE1 methyl ester in 0.8 g Witepsol S-52) were 100-350 pg/ml i.e. in the same order of magnitude as earlier seen for 16,16-dimethyl-PGE2.

• Research Organization: Karolinska Hospital, Stockholm, Sweden
• OSTI ID: 7008163
• Journal Information: Prostaglandins; (United States), Vol. 25:2
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
DEUTERIUM COMPOUNDS
GAS CHROMATOGRAPHY
MASS SPECTROSCOPY
PROSTAGLANDINS
BLOOD PLASMA
PATIENTS
PREGNANCY
BIOLOGICAL MATERIALS
BLOOD
BODY FLUIDS
CHROMATOGRAPHY
HYDROGEN COMPOUNDS
MATERIALS
SEPARATION PROCESSES
SPECTROSCOPY
550201* - Biochemistry- Tracer Techniques