Pathways of Cl sup minus transport in human fibroblasts

Journal Article · Fri Jul 01 04:00:00 EDT 1988 · American Journal of Physiology; (USA)

OSTI ID:7007865

Lin, P; Gruenstein, E ^[1]

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1. Univ. of Cincinnati College of Medicine, OH (USA)

Three pathways of Cl{sup {minus}} efflux were identified in normal human fibroblasts. Twenty percent of the total Cl{sup {minus}} efflux is via an electrically conductive pathway with an efflux constant of 0.016 min{sup {minus}1}. This pathway is insensitive to 4,4{prime}-diisothiocyanostilbene-2,2{prime}-disulfonic acid (DIDS) and bumetanide but is partially inhibited by anthracene-9-carboxylic acid. Twenty-five percent of the Cl{sup {minus}} efflux occurs via Cl{sup {minus}} with cation cotransport having an efflux constant of 0.020 min{sup {minus}1}. This pathway is inhibited by bumetanide and is dependent on the simultaneous presence of Na{sup +}, K{sup +}, and Cl{sup {minus}}. Under basal conditions, the energetics of this pathway indicate that it is operating close to equilibrium. Fifty percent of the Cl{sup {minus}} efflux occurs via an anion exchange pathway having an efflux constant of 0.040 min{sup {minus}1} that is inhibited by DIDS or by removal of Cl{sup {minus}} from the extracellular medium. Together these pathways account for 95% of the total Cl{sup {minus}} efflux.

• OSTI ID: 7007865
• Journal Information: American Journal of Physiology; (USA), Journal Name: American Journal of Physiology; (USA) Vol. 255:1; ISSN 0002-9513; ISSN AJPHA
• Country of Publication: United States
• Language: English

Related Subjects

551001* -- Physiological Systems-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALKALI METAL ISOTOPES
AMIDES
AMINO ACIDS
ANIMAL CELLS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL PATHWAYS
CARBON 14 COMPOUNDS
CARBONIC ACID DERIVATIVES
CARBOXYLIC ACIDS
CHLORINE 36
CHLORINE ISOTOPES
COMPARTMENTS
CONNECTIVE TISSUE CELLS
DAYS LIVING RADIOISOTOPES
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
EVEN-ODD NUCLEI
FIBROBLASTS
INTERMEDIATE MASS NUCLEI
ISOMERIC TRANSITION ISOTOPES
ISOTOPES
LABELLED COMPOUNDS
LIGHT NUCLEI
LIPOTROPIC FACTORS
MAMMALS
MAN
MEMBRANE TRANSPORT
METHIONINE
MINUTES LIVING RADIOISOTOPES
NUCLEI
ODD-ODD NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PHYSIOLOGY
PRIMATES
RADIOISOTOPES
RUBIDIUM 86
RUBIDIUM ISOTOPES
SECRETION
SOMATIC CELLS
SULFUR 35
SULFUR ISOTOPES
UPTAKE
UREA
VERTEBRATES
YEARS LIVING RADIOISOTOPES