Identification of genotoxic components of the heavy ends of energy-related materials

Technical Report · Thu Feb 01 04:00:00 EST 1990

DOI:https://doi.org/10.2172/7007668· OSTI ID:7007668

Lee, M. L. ^[1]; Castle, R. N. ^[2]

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1. Brigham Young Univ., Provo, UT (USA). Dept. of Chemistry
2. University of South Florida, Tampa, FL (USA). Dept. of Chemistry

The objective of this work was to develop capillary supercritical fluid chromatographic methods for the analysis of the high-molecular-weight components in high boiling, heavy and materials that are responsible for genotoxic activity. Since these compounds were largely unknown, 62 standard reference compounds were synthesized for comparison with actual high boiling coal-derived products. These compounds were comprised of two- and three-ring aromatic moieties coupled together with carbon, oxygen, sulfur, and nitrogen bridging groups. Mass spectral evidence indicated that a number of these compound types could be present in various coal products at a concentration level of 0.01--0.2 percent, although none of them could be positively identified because of the complexities of the fractions. A number of capillary supercritical fluid chromatographic instrumental developments were made as a result of this work. These include a supercritical fluid fractionation system for group-type separation of complex samples and a direct coupled supercritical fluid chromatograph/double focusing mass spectrometer system. Finally, a number of supercritical fluid chromatographic mobile phase combinations were evaluated for the analysis of large polycyclic aromatic compounds. 34 refs., 23 figs., 2 tabs.

• Research Organization: Brigham Young Univ., Provo, UT (USA). Dept. of Chemistry
• Sponsoring Organization: DOE/ER
• DOE Contract Number: FG02-86ER60445
• OSTI ID: 7007668
• Report Number(s): DOE/ER/60445-4; ON: DE90008373
• Country of Publication: United States
• Language: English

Related Subjects

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
400102* -- Chemical & Spectral Procedures
54 ENVIRONMENTAL SCIENCES
540120 -- Environment
Atmospheric-- Chemicals Monitoring & Transport-- (1990-)
AROMATICS
CHROMATOGRAPHY
DOCUMENT TYPES
FRACTIONATION
HYDROCARBONS
MASS SPECTROSCOPY
MOLECULAR WEIGHT
ORGANIC COMPOUNDS
PHYSICAL PROPERTIES
POLYCYCLIC AROMATIC HYDROCARBONS
PROGRESS REPORT
SEPARATION PROCESSES
SOLUBILITY
SPECTROSCOPY
SUPERCRITICAL FLUID CHROMATOGRAPHY
SYNTHESIS