Californium-252 plasma desorption with Fourier transform mass spectroscopy

Journal Article · Thu Jan 01 00:00:00 EST 1987 · Anal. Chem.; (United States)

DOI:https://doi.org/10.1021/ac00141a032· OSTI ID:7005740

Loo, J A; Williams, E R; Amster, I J; Furlong, J J.P.; Wang, B H; McLafferty, F W; Chait, B T; Field, F H

Plasma desorption (PD) such, as that induced by the 100-MeV fission products of /sup 252/Cf, is a particularly promising ionization method for large molecules, yielding molecular ion species even from trypsin, molecular weight 23,463. Further, with trypsin using nitrocellulose as the substrate, (M + 3H)/sup 3 +/ is the most abundant molecular ion species and (M + 6H)/sup 6 +/ is measurable, which greatly extends the mass values (m) observable for instruments with an upper m/z limit (z = number of charges). However, a 50-..mu..Ci /sup 252/Cf source only produces 55,000 fissions s/sup -1/, yielding ion currents that are generally much too low for scanning instruments. An instrument with unusual capabilities for these, as well as for simultaneous ion detection over a wide mass range, is the Fourier transform (FT) mass spectrometer. With FTMS, Hunt has measured (M + H)/sup +/ ions of cytochrome c, molecular weight 12,384, ionized in an exterior fast-atom-bombardment source. Here the authors describe techniques for obtaining PD/FT mass spectra for a variety of compounds with abundant molecular ion species of masses as high as 2016 (alamethicin).

• Research Organization: Cornell Univ., Ithaca, NY (USA)
• OSTI ID: 7005740
• Journal Information: Anal. Chem.; (United States), Vol. 59
• Country of Publication: United States
• Language: English

Related Subjects

37 INORGANIC
ORGANIC
PHYSICAL AND ANALYTICAL CHEMISTRY
59 BASIC BIOLOGICAL SCIENCES
BRADYKININ
DESORPTION
MASS SPECTROSCOPY
ION SOURCES
PERFORMANCE
VITAMIN B-12
CALIFORNIUM 252
FISSION PRODUCT RELEASE
FOURIER TRANSFORM SPECTROMETERS
IONIZATION
MASS SPECTRA
QUALITATIVE CHEMICAL ANALYSIS
ACTINIDE ISOTOPES
ACTINIDE NUCLEI
ALPHA DECAY RADIOISOTOPES
CALIFORNIUM ISOTOPES
CHEMICAL ANALYSIS
DRUGS
EVEN-EVEN NUCLEI
HEAVY NUCLEI
HEMATINICS
HEMATOLOGIC AGENTS
ISOTOPES
KININS
MEASURING INSTRUMENTS
NUCLEI
ORGANIC COMPOUNDS
PEPTIDES
POLYPEPTIDES
PROTEINS
RADIOISOTOPES
SPECTRA
SPECTROMETERS
SPECTROSCOPY
VITAMIN B GROUP
VITAMINS
YEARS LIVING RADIOISOTOPES
400102* - Chemical & Spectral Procedures
550200 - Biochemistry