Californium-252 plasma desorption with Fourier transform mass spectroscopy

Journal Article · Wed Dec 31 23:00:00 EST 1986 · Anal. Chem.; (United States)

DOI:https://doi.org/10.1021/ac00141a032· OSTI ID:7005740

Loo, J A; Williams, E R; Amster, I J; Furlong, J J.P.; Wang, B H; McLafferty, F W; Chait, B T; Field, F H

Plasma desorption (PD) such, as that induced by the 100-MeV fission products of /sup 252/Cf, is a particularly promising ionization method for large molecules, yielding molecular ion species even from trypsin, molecular weight 23,463. Further, with trypsin using nitrocellulose as the substrate, (M + 3H)/sup 3 +/ is the most abundant molecular ion species and (M + 6H)/sup 6 +/ is measurable, which greatly extends the mass values (m) observable for instruments with an upper m/z limit (z = number of charges). However, a 50-..mu..Ci /sup 252/Cf source only produces 55,000 fissions s/sup -1/, yielding ion currents that are generally much too low for scanning instruments. An instrument with unusual capabilities for these, as well as for simultaneous ion detection over a wide mass range, is the Fourier transform (FT) mass spectrometer. With FTMS, Hunt has measured (M + H)/sup +/ ions of cytochrome c, molecular weight 12,384, ionized in an exterior fast-atom-bombardment source. Here the authors describe techniques for obtaining PD/FT mass spectra for a variety of compounds with abundant molecular ion species of masses as high as 2016 (alamethicin).

• Research Organization: Cornell Univ., Ithaca, NY (USA)
• OSTI ID: 7005740
• Journal Information: Anal. Chem.; (United States), Journal Name: Anal. Chem.; (United States) Vol. 59; ISSN ANCHA
• Country of Publication: United States
• Language: English

Related Subjects

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
400102* -- Chemical & Spectral Procedures
550200 -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
ACTINIDE ISOTOPES
ACTINIDE NUCLEI
ALPHA DECAY RADIOISOTOPES
BRADYKININ
CALIFORNIUM 252
CALIFORNIUM ISOTOPES
CHEMICAL ANALYSIS
DESORPTION
DRUGS
EVEN-EVEN NUCLEI
FISSION PRODUCT RELEASE
FOURIER TRANSFORM SPECTROMETERS
HEAVY NUCLEI
HEMATINICS
HEMATOLOGIC AGENTS
ION SOURCES
IONIZATION
ISOTOPES
KININS
MASS SPECTRA
MASS SPECTROSCOPY
MEASURING INSTRUMENTS
NUCLEI
ORGANIC COMPOUNDS
PEPTIDES
PERFORMANCE
POLYPEPTIDES
PROTEINS
QUALITATIVE CHEMICAL ANALYSIS
RADIOISOTOPES
SPECTRA
SPECTROMETERS
SPECTROSCOPY
VITAMIN B GROUP
VITAMIN B-12
VITAMINS
YEARS LIVING RADIOISOTOPES