Removal of nonhomologous DNA ends in double-strand break recombination: The role of the yeast ultraviolet repair gene RAD1

Journal Article · Thu Oct 15 04:00:00 EDT 1992 · Science (Washington, D.C.); (United States)

DOI:https://doi.org/10.1126/science.1411547· OSTI ID:7005585

Fishman-Lobell, J; Habert, J E ^[1]

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1. Brandeis Univ., Waltham, MA (United States)

Double-strand breaks (DSBs) in Saccharomyces cerevisiae can be repaired by gene conversions or by deletions resulting from single-strand annealing between direct repeats of homologous sequences. Although rad1 mutants are resistant to x-rays and can complete DSB-mediated mating-type switching, they could not complete recombination when the ends of the break contained approximately 60 base pairs of nonhomology. Recombination was restored when the ends of the break were made homologous to donor sequences. Additionally, the absence of RAD1 led to the frequent appearance of a previously unobserved type of recombination product. These data suggest RAD1 is required to remove nonhomologous DNA from the 3{prime} ends of recombining DNA, a process analogous to the excision of photodimers during repair of ultraviolet-damaged DNA.

• OSTI ID: 7005585
• Journal Information: Science (Washington, D.C.); (United States), Journal Name: Science (Washington, D.C.); (United States) Vol. 258:5081; ISSN SCIEA; ISSN 0036-8075
• Country of Publication: United States
• Language: English

Related Subjects

560130* -- Radiation Effects on Microorganisms
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
DNA REPAIR
ELECTROMAGNETIC RADIATION
EUMYCOTA
FUNGI
GENE RECOMBINATION
GENES
MICROORGANISMS
PLANTS
RADIATION EFFECTS
RADIATIONS
REPAIR
SACCHAROMYCES
SACCHAROMYCES CEREVISIAE
STRAND BREAKS
ULTRAVIOLET RADIATION
YEASTS