S-phosphocysteine and phosphohistidine are intermediates in the phosphoenolpyruvate-dependent mannitol transport catalyzed by Escherichia coli EII/sup Mtl/
During a cycle of mannitol transport and phosphorylation, the phosphoryl group originating on P-enolpyruvate is transferred, consecutively, to two sites on the Escherichia coli mannitol-specific carrier (EII/sup Mtl/) before being placed on mannitol. The peptides constituting the two EII/sup Mtl/ phosphorylation sites have been isolated and identified after labeling with (/sup 32/P)-P-enolpyruvate. The first site is localized in peptide Leu 541-Lys 560. The hydrolysis characteristics of the phosphorylated peptide indicate that a histidine residue is phosphorylated. The second site is located in peptide Ile 380-Met 393, which contains the activity-linked cysteine (384). The hydrolysis characteristics of the phosphopeptide indicate that Cys 384 is the site of phosphorylation.
- Research Organization:
- Univ. of Groningen (Netherlands)
- OSTI ID:
- 7002727
- Journal Information:
- Biochemistry; (United States), Journal Name: Biochemistry; (United States) Vol. 27:16; ISSN BICHA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
ALKALINE PHOSPHATASE
AMINO ACID SEQUENCE
AMINO ACIDS
AZOLES
BACTERIA
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CARBOXYLIC ACIDS
CHEMICAL REACTIONS
CHROMATOGRAPHY
CYSTEINE
DAYS LIVING RADIOISOTOPES
DECOMPOSITION
ENZYMATIC HYDROLYSIS
ENZYMES
ESCHERICHIA COLI
ESTERASES
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
HISTIDINE
HYDROLASES
HYDROLYSIS
IMIDAZOLES
ISOTOPES
LABELLED COMPOUNDS
LIGHT NUCLEI
LIQUID COLUMN CHROMATOGRAPHY
LYSIS
MICROORGANISMS
MOLECULAR STRUCTURE
NUCLEI
ODD-ODD NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PEPTIDES
PH VALUE
PHOSPHATASES
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PHOSPHORYLATION
PROTEINS
RADIOISOTOPES
SEPARATION PROCESSES
SOLVOLYSIS
THIOLS