Metabolism of platelet activating factor at the whole organ and cellular level
Thesis/Dissertation
·
OSTI ID:7001959
Platelet activating factor (PAF, 1-O-alkyl-2-acetyl-sn-3-glycerophosphocholine) has been characterized as a phospholipid possessing a myriad of effects from the cellular to whole organism levels. Analytical methods and procedures were developed in order to measure and identify PAF precursors and metabolites. Two quantitative physicochemical methods based on isotope dilution mass spectrometry (MS) were developed to measure lyso-PAF and applied to the calcium ionophore stimulated human neutrophil. Levels of lyso-PAF were found to be significantly increased, 2-3 fold, upon cell activation with a stimulus that concomitantly elicits the production of PAF. Investigation into the metabolism of PAF by the isolated perfused rat lung by intratracheal instillation revealed (/sup 3/H)-PAF to be extensively metabolized over a 15 minute time course. Greater than 96% of the administered dose was retained by the lung and was distributed as: lyso-PAF (3.3%), phosphatidylcholine (GPC, 82.3%), phosphatidylethanolamine (2.5%), and neutral lipid (2.5%), the remainder was intact PAF.
- Research Organization:
- Colorado Univ., Denver (USA). Health Sciences Center
- OSTI ID:
- 7001959
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550501* -- Metabolism-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BLOOD COAGULATION FACTORS
BODY
BODY FLUIDS
COAGULANTS
DRUGS
ESTERS
HEMATOLOGIC AGENTS
ISOTOPE APPLICATIONS
ISOTOPE DILUTION
LABELLED COMPOUNDS
LEUKOCYTES
LIPIDS
LUNGS
MAMMALS
MAN
MASS SPECTROSCOPY
MATERIALS
METABOLISM
METABOLITES
NEUTROPHILS
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
ORGANS
PERFUSED ORGANS
PHOSPHOLIPIDS
PRECURSOR
PRIMATES
RATS
RESPIRATORY SYSTEM
RODENTS
SPECTROSCOPY
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VERTEBRATES
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BLOOD COAGULATION FACTORS
BODY
BODY FLUIDS
COAGULANTS
DRUGS
ESTERS
HEMATOLOGIC AGENTS
ISOTOPE APPLICATIONS
ISOTOPE DILUTION
LABELLED COMPOUNDS
LEUKOCYTES
LIPIDS
LUNGS
MAMMALS
MAN
MASS SPECTROSCOPY
MATERIALS
METABOLISM
METABOLITES
NEUTROPHILS
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
ORGANS
PERFUSED ORGANS
PHOSPHOLIPIDS
PRECURSOR
PRIMATES
RATS
RESPIRATORY SYSTEM
RODENTS
SPECTROSCOPY
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VERTEBRATES