Cloning of large segments of exogenous DNA into yeast by means of artificial chromosome vectors

Journal Article · Mon May 11 04:00:00 EDT 1987 · Science (Washington, D.C.); (United States)

DOI:https://doi.org/10.1126/science.3033825· OSTI ID:7000234

Burke, D T; Carle, G F; Olson, M V

Fragments of exogenous DNA that range in size up to several hundred kilobase pairs have been cloned into yeast by ligating them to vector sequences that allow their propagation as linear artificial chromosomes. Individual clones of yeast and human DNA that have been analyzed by pulsed-field gel electrophoresis appear to represent faithful replicas of the source DNA. The efficiency with which clones can be generated is high enough to allow the construction of comprehensive libraries from the genomes of higher by offering a tenfold in the size of the DNA molecules that can be cloned microbial host, this system addresses a major gap in existing experimental methods for analyzing complex DNA sources.

• Research Organization: Washington Univ. School of Medicine, St. Louis, MO (USA)
• OSTI ID: 7000234
• Journal Information: Science (Washington, D.C.); (United States), Journal Name: Science (Washington, D.C.); (United States) Journal Issue: 4803 Vol. 236; ISSN SCIEA
• Country of Publication: United States
• Language: English

Related Subjects

550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
CHROMOSOMES
CLONING
DNA
DNA REPLICATION
DNA-CLONING
ELECTROPHORESIS
FUNGI
MICROORGANISMS
NUCLEIC ACID REPLICATION
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PLANTS
RECOMBINANT DNA
YEASTS